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首页> 外文期刊>African Journal of Biotechnology >Co-expression of apoptin (VP3) and antibacterial peptide cecropin B mutant (ABPS1) genes induce higher rate of apoptosis in HepG2 and A375 cell lines
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Co-expression of apoptin (VP3) and antibacterial peptide cecropin B mutant (ABPS1) genes induce higher rate of apoptosis in HepG2 and A375 cell lines

机译：Apoptin（VP3）和抗菌肽crocropin B突变体（ABPS1）基因的共表达诱导HepG2和A375细胞系凋亡的速率更高

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The?antibacterial peptide cecropin B mutant (ABPS1) gene has a broad range of antibacterial and antiproliferative properties. Apoptin (VP3), a chicken anaemia virus-encoded protein is known to induce apoptosis in human transformed cells.?To explore drug combination in human tumor cells, apoptin and ABPS1 eukaryotic expression vector pIRES2-EGFP-apoptin and pIRES2-EGFP-ABPS1 were constructed and their expression effect individually and in combinations were studied in HepG2 and A375 cells. The vector pIRES2-EGFP-ABPS1 and pIRES2-EGFP-apoptin were transfected into tumor cells HepG2 and A375 by the lipofectamine-mediated DNA transfection procedure. At 48 h post transfection, the apoptotic rate obtained by flow cytometry and the morphological changes under light and scanning electron microscope of tumor cells were significant. In contrast, the microvilli on the surface of the control cells were disrupted, decreased and even disappeared. The cell membrane was injured and intracellular substances leaked out. Furthermore, our results indicate that the apoptotic rates of apoptin (27.32% in HepG2 and 9.34% in A375 cells), were higher than ABPS1 (23.79% in HepG2 and 8.33% in A375 cells). Moreover, the co-expression of Apoptin and ABPS1 showed higher apoptotic rates which were 27.66 and 10.33% in HepG2 and A375 cells respectively. However, the apoptotic rates obtained in HepG2 cells treated with apoptin and apoptin and ABPS1 together were closely similar, but, not in A375 cells. Therefore, the results of the present study showed that the combination of Apoptin and ABPS1 has synergistic effect in HepG2 and A375 cell lines.?Keys words:?Apoptin, ABPS1, apoptosis, co-expression, HepG2, A375.
机译：抗菌肽天蚕素B突变体（ABPS1）基因具有广泛的抗菌和抗增殖特性。已知鸡贫血病毒编码蛋白Apoptin（VP3）诱导人转化细胞凋亡。为了探索人肿瘤细胞中的药物组合，Apoptin和ABPS1真核表达载体pIRES2-EGFP-apoptin和pIRES2-EGFP-ABPS1分别存在。在HepG2和A375细胞中研究了它们的构建及其单独或组合的表达效果。通过脂转染胺介导的DNA转染程序将载体pIRES2-EGFP-ABPS1和pIRES2-EGFP-细胞凋亡素转染到肿瘤细胞HepG2和A375中。转染后48 h，流式细胞仪检测到的凋亡率以及光和扫描电镜下肿瘤细胞的形态变化均很显着。相反，对照细胞表面的微绒毛被破坏，减少甚至消失。细胞膜受损，细胞内物质泄漏出去。此外，我们的结果表明，凋亡素的凋亡率（HepG2中27.32％，A375细胞中9.34％）高于ABPS1（HepG2中23.79％，A375细胞8.33％）。此外，Apoptin和ABPS1的共表达显示出较高的凋亡率，分别在HepG2和A375细胞中分别为27.66％和10.33％。然而，在用凋亡素和凋亡素与ABPS1一起处理的HepG2细胞中获得的凋亡率非常相似，但是在A375细胞中却没有。因此，本研究结果表明，Apoptin和ABPS1的组合在HepG2和A375细胞系中具有协同作用。关键词：Apoptin，ABPS1，凋亡，共表达，HepG2，A375。

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