• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Mutational analysis of fructose-1,6-bisphosphate aldolase of Neisseria meningitidis serogroup B
【24h】

Mutational analysis of fructose-1,6-bisphosphate aldolase of Neisseria meningitidis serogroup B

机译：脑膜炎奈瑟菌B群血清果糖1，，6-二磷酸醛缩酶的突变分析

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Fructose-1,6-bisphosphate aldolase (FBA) is a classical cytoplasmic glycolytic enzyme which, despite lacking a predicted signal peptide, has been demonstrated to be expressed and transported to the surface of numerous Gram-positive bacteria and shown to interact with host molecules and perform non-glycolytic biological functions. Genome-based studies have also demonstrated that the glycolytic pathway appears to be non-functional in the meningococcus due to absence of phosphofructokinase, one of the important enzymes in this pathway. This study aimed to investigate whether the FBA, a so-called housekeeping enzyme, is required for maximal?in vitrogrowth of?N. meningitidis. An FBA knock-out mutant was created in?N. meningitidisusing an inverse polymerase chain reaction (PCR) and allelic exchange methodology. Phenotypic analysis of FBA-deficient mutant strains such as growth profiling experiments showed that the FBA-deficient mutant grew at the same rate (in broth culture and on solid media) as the wild-type strain, suggesting that FBA is not required for optimal growth of?N. meningitidis?under the?in vitro?conditions tested. No differences in either colony or bacterial cell morphology (using light microscopy) were observed. In summary, despite being a central enzyme in the glycolytic cycle, FBA is not required for?in vitro?growth of?N. meningitidis.
机译：1,6-双磷酸果糖醛缩酶（FBA）是一种经典的细胞质糖酵解酶，尽管缺乏预测的信号肽，但已被证明可表达并转运至多种革兰氏阳性细菌的表面，并显示出与宿主分子的相互作用并执行非糖酵解生物学功能。基于基因组的研究还表明，由于缺乏果糖磷酸激酶（该途径中的重要酶之一），因此糖酵解途径在脑膜炎球菌中似乎无功能。这项研究旨在调查是否需要FBA，即所谓的管家酶，以最大程度地增加ΔN的体外生长。脑膜炎。在？N中创建了一个FBA敲除突变体。脑膜炎使用反向聚合酶链反应（PCR）和等位基因交换方法。 FBA缺陷型突变株的表型分析（例如生长谱实验）显示，FBA缺陷型突变株以与野生型菌株相同的速率（在肉汤培养中和在固体培养基上）生长，这表明最佳生长不需要FBA的？脑膜炎在体外条件下测试。在菌落或细菌细胞形态（使用光学显微镜）中均未观察到差异。总之，尽管在糖酵解周期中是核心酶，但FBA并不是“ N”的体外生长所必需的。脑膜炎。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号