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首页> 外文期刊>African Journal of Biotechnology >Molecular mechanism of methionine differentiation in high and low methionine maize lines
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Molecular mechanism of methionine differentiation in high and low methionine maize lines

机译：高低蛋氨酸玉米品系蛋氨酸分化的分子机制。

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Since maize is a primary food stuff for humans and livestock, its amino acid balance is important for proper nutrition. Methionine, an essential amino acid and a primary source of sulfur, is lacking in maize endosperm. Several maize populations were developed through breeding with enhanced methionine content in comparison with normal maize populations. BS31HM?(high methionine)?and BS31LM?(low methionine)?maize were among such populations created by the selection from the highest or lowest methionine content population from original BS31 maize. Candidate gene approach was adopted to determine the difference between the two populations at transcript level of the selected genes in the endosperm. The genes selected were mostly expressed in the endosperm and could be involved in enhanced methionine biosynthesis. The selected genes, that is, 15-kDa β-zein, 16-kDa γ-zein, 19-kDa α-zeinB1, 27-kDa γ-zein, 22-kDa α-zein and 18-kDa δ-zein were responsible for coding of endosperm storage proteins when analyzed through RT-PCR. Similarly, expression level relative to the high population (2-ΔΔct) values were also calculated for BS31HM and BS31LM, respectively. These? values were found as 1 and 0.25, 1 and 0.07, 1 and 0.10, 1 and 0.15, 1 and 0.33, 1 and 0.43 for 27-kDa γ-zein, 22-kDa α-zein, 18-kDa δ-zein, 15-kDa β-zein, 16-kDa γ-zein and 19-kDa α-zeinB1, respectively, in both populations. The p-values were determined by student’s t-test at confidence level of 95%. The expression of 18-kDa δ-gene, 15-kDa β-gene and 16-kDa γ-gene were found to be significant (p 0.05) differences in the expression level of 27-kDa γ-gene, 22-kDa α-gene and 19-kDa α-gene were observed in both HM and LM maize populations. From these results it can be concluded that all zein genes did not show expression equally in high and low methionine maize populations.
机译：由于玉米是人类和牲畜的主要食品，因此其氨基酸平衡对于适当的营养至关重要。玉米胚乳缺乏蛋氨酸，一种必需氨基酸，也是硫的主要来源。通过育种，与正常玉米种群相比，蛋氨酸含量提高了一些玉米种群。通过从原始BS31玉米的最高或最低蛋氨酸含量种群中进行选择来创建BS31HMα（高蛋氨酸）和BS31LM（低蛋氨酸）玉米。采用候选基因方法来确定两个群体在胚乳中所选基因的转录水平上的差异。选择的基因主要在胚乳中表达，并可能参与增强蛋氨酸的生物合成。所选择的基因，即15-kDaβ-玉米醇溶蛋白，16-kDaγ-玉米醇溶蛋白，19-kDaα-玉米醇溶蛋白B1、27-kDaγ-玉米醇溶蛋白，22-kDaα-玉米醇溶蛋白和18-kDaδ-玉米醇溶蛋白负责。 RT-PCR分析时用于胚乳贮藏蛋白编码的方法。类似地，还分别针对BS31HM和BS31LM计算了相对于高群体（2-ΔΔct）值的表达水平。这些？ 27-kDaγ-玉米醇溶蛋白，22-kDaα-玉米醇溶蛋白，18-kDaδ-玉米醇溶蛋白的值分别为1和0.25、1和0.07、1和0.10、1和0.15、1和0.33、1和0.43 -kDaβ-玉米醇溶蛋白，16-kDaγ-玉米醇溶蛋白和19-kDaα-玉米醇溶蛋白B1。 p值由学生的t检验确定，置信度为95％。发现18-kDaδ基因，15-kDaβ基因和16-kDaγ基因的表达在27-kDaγ基因，22-kDaα-的表达水平上有显着性差异（p 0.05）。在HM和LM玉米群体中均观察到了KnO基因和19-kDaα基因。从这些结果可以得出结论，在高和低甲硫氨酸玉米群体中，所有玉米醇溶蛋白基因均未显示相同的表达。

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