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Cloning and analysis of plant fatty acid desaturase 7 gene promoter from Brassica napus

机译:甘蓝型油菜植物脂肪酸去饱和酶7基因启动子的克隆与分析

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n order to investigate the regulation mode of?Brassica napus?FAD7?gene in response of thermal stress, we measured the protein levels of?BnFAD7?in?plant at low and high temperature, and then analyzed promoter activity of?5’-flanking regions of?BnFAD7?by transient gene expression in?B. napus?protoplasts at different temperatures. Our studies indicated that no significant change occurred in the expression level ofBnFAD7?both at high and low temperature, while?BnFAD7?promoter showed a heat-induced regulation mode and slowly increased activity at the chilling conditions, which suggested there are heat-induced cis-action element lies in?BnFAD7?promoter sequence. Our data also suggested that a post-transcription regulation pattern existed to ensure BnFAD7 function in the?acclimation to temperature stress. Furthermore, our studies give new evidence for the hypothesis that?BnFAD7?and?BnFAD8?gene may come from the same ancestor gene.
机译:为了研究甘蓝型油菜FAD7基因在热胁迫下的调控模式,我们测量了低温条件下植物中BnFAD7蛋白的蛋白水平,然后分析了5'侧翼启动子的活性。通过瞬时基因表达在βB中的“ BnFAD7”区域。甘蓝型原生质体在不同温度下。我们的研究表明,在高温和低温下,BnFAD7β的表达水平均未发生显着变化,而“BnFAD7α”的启动子则表现出热诱导的调节模式,并且在寒冷条件下的活性缓慢增加,这表明存在热诱导的顺式-action元素位于“ BnFAD7”启动子序列中。我们的数据还表明,存在转录后调控模式以确保BnFAD7在适应温度胁迫中发挥功能。此外,我们的研究为“ BnFAD7”和“ BnFAD8”基因可能来自同一祖先基因这一假说提供了新的证据。

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