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首页> 外文期刊>African Journal of Pure and Applied Chemistry >Spectrophotmetric evaluation of rotenone extraction from leaves and seeds of mature Tephrosia vogelii plant
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Spectrophotmetric evaluation of rotenone extraction from leaves and seeds of mature Tephrosia vogelii plant

机译：分光光度法评价伏牛藤成熟植物叶片和种子中鱼藤酮的提取

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摘要

The percentage yield of rotenone extracted from Tephrosia vogelii leaves and seeds was investigated. Ten samples of well ground leaves and seeds were each subjected to extraction in soxhlet extraction system using trichloromethane, ethanol and methanol solvents for 24 h at room temperature. The maximum absorption wavelength of rotenone was determined using Ultra violet-visible spectrophotometer. Different solvent extracts were quantified using high performance liquid chromatography instruments fitted with Ultra Violet detector and their yield expressed as percentage rotenone. Trichloromethane recorded the highest rotenone yield from both leaves (8.3 and seeds 2.7%) compared to the other two solvents. Ethanol was second with 5.9% in leaves and 1.9% in seeds while methanol had 4.8% in leaves and 1.6% in seeds. In general, the leaves extracts were found to have a higher rotenone percentage yield by an average factor of 3 compared to seeds. Rotenone can be commercially extracted from T. Vogelii using ethanol that is locally available to complement natural pyrethrum insecticide industry.
机译：研究了从佛手叶和种子中提取的鱼藤酮的百分率。在室温下，使用三氯甲烷，乙醇和甲醇溶剂，在索氏提取系统中对十份磨碎的叶子和种子样品进行索氏提取系统的提取，历时24小时。使用紫外可见分光光度计测定鱼藤酮的最大吸收波长。使用装有紫外检测器的高效液相色谱仪对不同的溶剂提取物进行定量，其产率以鱼藤酮的百分比表示。与其他两种溶剂相比，三氯甲烷从两片叶子（8.3和种子2.7％）中记录到最高的鱼藤酮收率。乙醇次之，其叶子为5.9％，种子为1.9％，而甲醇的叶子为4.8％，种子为1.6％。通常，与种子相比，发现叶提取物的鱼藤酮百分率更高，平均因子为3。鱼藤酮可使用当地可得来补充天然除虫菊杀虫剂工业的乙醇从T. Vogelii中商业提取。

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