• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Agriculture, Forestry and Fisheries >Field Assessment of Agronomic Performance, Resistance to Aflatoxin, and Fumonisin Accumulation in Selected Maize Inbred Lines in Kenya
【24h】

Field Assessment of Agronomic Performance, Resistance to Aflatoxin, and Fumonisin Accumulation in Selected Maize Inbred Lines in Kenya

机译：肯尼亚部分玉米自交系农艺表现，对黄曲霉毒素的抗性和伏马菌素积累的田间评估

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Aspergillus flavus and Fusarium verticilloides cause contamination of maize (Zea mays) and concern to maize farmers because they reduce maize quantity and quality. These fungi produce mycotoxins, some of which are poisonous to both humans and animals. Over 300 mycotoxins are known but in this study aflatoxins and fumonisins produced by A. flavus and F. verticilloides, respectively, are reported due to their health concerns in Africa. Contamination of maize grain by these fungi occurs before harvest and selection of maize parental lines resistant to accumulation of aflatoxin, and fumonisin for breeding purposes is the easiest strategy to reduce consumption of maize grains contaminated by these toxins. In addition to selecting for resistant lines, breeders would prefer inbred lines with top performing agronomic traits. This study aimed at identifying possible source of resistance together with good agronomic traits among 23 maize inbred lines (13 sourced from the MAIZE Competitive Grants Initiative, International Maize and Wheat Improvement Centre and 10 from Agricultural Research Council, South Africa). The lines were planted in two blocks; Aspergillus, and Fusarium, in a randomized complete-block design for two seasons in one location in Kenya. Germination rate, days to silking, and days to anthesis were determined in each line. Inoculation of the maize ears was done at silking with three toxigenic strains of A. flavus for Aspergillus block, and F. verticilloides for Fusarium block. Aflatoxins and fumonisins concentration in the kernels was determined using Enzyme-linked immunosorbent assay (ELISA). A positive significant correlation (r = 0.9458846, P = 9.845e-12) occurred between days to anthesis and days to silking, aflatoxins and fumonisins (r = 0.43149988, P ≤ 0.05) accumulated in the inbred lines. A negative correlation between germination and accumulated fumonisin levels (r = -0.5156961, P = 0.01178), days to pollen shed and aflatoxin (r = -0.4617732, P = 0.02654) was revealed. Apart from being good germinating lines and drought tolerant, CML 390 and CML 247 accumulated least fumonisin, and aflatoxin levels compared to the other germplasms. These two lines with consistent low aflatoxin, and fumonisin levels may, therefore be useful sources of resistance for maize breeding programs to reduce both aflatoxin and fumonisn contamination in maize. Four aflatoxin resistant lines (CB 222, CML 495 and CML 444) and one (CKL05003) fumonisin resistant line showed good agronomic traits. The lines may be suitable for breeding for resistance to aflatoxins, and fumonisins respectively in maize.
机译：黄曲霉和镰刀菌引起玉米污染（玉米（Zea mays）），并引起玉米种植者的关注，因为它们降低了玉米的数量和质量。这些真菌产生霉菌毒素，其中一些毒素对人类和动物都有毒。已知有300多种霉菌毒素，但在本研究中，由于非洲地区的健康问题，分别报道了黄曲霉和黄萎病菌产生的黄曲霉毒素和伏马毒素。这些真菌对玉米籽粒的污染发生在收割之前，并且选择对黄曲霉毒素积累有抵抗力的玉米亲本品系，而伏马菌素用于育种是减少被这些毒素污染的玉米籽粒消耗的最简单策略。除选择抗性品系外，育种者更喜欢具有最佳农艺性状的近交品系。这项研究旨在确定23个玉米自交系中可能的抗性来源以及良好的农艺性状（13个来自国际玉米和小麦改良中心MAIZE竞争性赠款计划，南非农业研究理事会提供10个）。将品系分两块种植;曲霉菌和镰刀菌属，在肯尼亚的一个地点进行了两个季节的随机完整区组设计。确定每行的发芽率，发丝天数和花期。玉米穗的接种是在蚕丝时用三种产毒菌株黄曲霉（A. flavus）用于曲霉菌块菌，以及黄萎病菌（F. verticilloides）用于镰刀菌属块菌。使用酶联免疫吸附测定（ELISA）测定籽粒中的黄曲霉毒素和伏马毒素含量。在自交系到开花期之间和到花丝，黄曲霉毒素和伏马毒素的天数之间存在正显着的相关性（r = 0.9458846，P = <9.845e-12）（r = 0.43149988，P≤0.05）。揭示了发芽与积累的伏马菌素水平（r = -0.5156961，P = 0.01178），花粉脱落天数和黄曲霉毒素（r = -0.4617732，P = 0.02654）之间呈负相关。除了具有良好的发芽系和耐旱性外，与其他种质相比，CML 390和CML 247积累的伏马毒素和黄曲霉毒素含量最少。因此，这两个品系始终具有较低的黄曲霉毒素和伏马毒素含量，可能是玉米育种计划中减少黄曲霉毒素和伏马毒素污染的有用抗性来源。四个抗黄曲霉毒素的品系（CB 222，CML 495和CML 444）和一个抗烟曲霉素的品系（CKL05003）表现出良好的农艺性状。该品系可能适合于分别对玉米中的黄曲霉毒素和伏马毒素进行抗性育种。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号