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首页> 外文期刊>Journal of Crop Improvement >Field evaluation of resistance to aflatoxin accumulation in maize inbred lines in Kenya and South Africa
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Field evaluation of resistance to aflatoxin accumulation in maize inbred lines in Kenya and South Africa

机译:肯尼亚与南非玉米自交系中黄曲霉毒素累积抗性的现场评价

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Aflatoxin, a carcinogenic toxin, is produced mainly by Aspergillus flavus and Aspergillus parasiticus. Contamination of maize (Zea mays L.) grain by these fungi occurs before harvest, and the easiest strategy to prevent this is to develop/use maize varieties resistant to Aspergillus spp. and aflatoxin accumulation. The objective of this investigation was to identify potential sources of resistance among 23 maize inbred lines (13 obtained from the MAIZE Competitive Grants Initiative, International Maize and Wheat Improvement Centre and 10 from Agricultural Research Council, South Africa). The inbred lines were planted in a randomized complete-block design at two locations each in Kenya and South Africa. Maize ears were inoculated at silking with three toxigenic strains of A. flavus. The inoculated ears in each plot were harvested at 12-18% moisture, dried, and visually assessed for Aspergillus ear rot (AER). Aflatoxin concentration in the kernels was determined using liquid chromatography-tandem mass spectrometry. Significant variation for both AER and aflatoxin concentration existed among the inbred lines at both locations in Kenya and one location in South Africa. Combined analysis revealed a significant (p < 0.001) lines x locations interactionfor both AER and aflatoxin concentration. Higher incidences of AER (0-86.0%) and aflatoxin concentration (0.21-6.51 ug/kg) were recorded at Kiboko in Kenya than at the other three locations. A stronger genetic correlation (rG = 0.936, p < 0.0001) betweenthe AER and aflatoxin concentration was recorded in Potchefstroom than at the other three locations. Repeatability of aflatoxin concentration was high at Kiboko (0.87) and Potchefstroom in South Africa (0.74). Three inbred lines, CML247, CML444, and CML495, emerged as potentially useful sources of resistance to AER and aflatoxin accumulation as they showed low levels of aflatoxin contamination in both localities in Kenya and in South Africa.
机译:致癌毒素的黄曲霉毒素主要由曲霉和曲霉和曲霉菌寄生产生。通过这些真菌在收获前发生玉米(Zea mays L.)谷物的污染,以及预防这一点的最简单的策略是开发/使用抗曲霉SPP的玉米品种。和黄曲霉毒素积累。该调查的目的是识别23米血统自交系中潜在的抵抗来源(从玉米竞争助资倡议,国际玉米和小麦改善中心和南非农业研究委员会的10个)。在肯尼亚和南非的两个地区的两个地区以随机的完整块设计种植了自交系。玉米耳朵被蚕丝用三种毒素的A.FlaVus接种。将每个图中的接种耳朵在12-18%的水分下收获,干燥,并视觉评估曲霉(AER)。使用液相色谱 - 串联质谱法测定核中的黄曲霉毒素浓度。在肯尼亚的两个地点和南非的一个地点存在近交系中的AER和黄曲霉毒素浓度的显着变化。组合分析显示了一种显着的(P <0.001)串行X位置,必须相互作用,这两种Aer和黄曲霉毒素浓度。在KIBOKO在肯尼亚的kiboko比其他三个位置记录较高的AER(0-86.0%)和黄曲霉毒素浓度(0.21-6.51ug / kg)的发病率。在PoTchefsthem中记录了比Aer和黄曲霉毒素浓度的更强的遗传相关性(RG = 0.936,P <0.0001)比在另一个三个位置。在Kiboko(0.87)和南非的Potchefshe(0.74)的kiboko(0.87)的可重复性高。三条自交系,CML247,CML444和CML495出现为潜在的抗病源和黄曲霉毒素积累,因为它们在肯尼亚和南非的地方显示出低水平的黄曲霉毒素污染。

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