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首页> 外文期刊>AIMS Genetics >The possible function of Flp1 in homologous recombination repair in Saccharomyces cerevisiae
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The possible function of Flp1 in homologous recombination repair in Saccharomyces cerevisiae

机译：Flp1在酿酒酵母同源重组修复中的可能作用

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Saccharomyces cerevisiae Mus81 is a structure-selective endonuclease which constitutes an alternative pathway in parallel with the helicase-topoisomerase Sgs1-Top3-Rmi1 complex to resolve a number of DNA intermediates during DNA replication, repair, and homologous recombination. Previously, it was showed that the N-terminal region of Mus81 was required for its in vivo function in a redundant manner with Sgs1; mus81Δ120N mutant that lacks the first 120 amino acid residues at the N-terminus exhibited synthetic lethality in combination with the loss of SGS1 . In this study, the physiologically important role of the N-terminal region of Mus81 in processing toxic intermediates was further investigated. We examined the cellular defect of sgs1Δmus81Δ100N cells and observed that although viable, the cells became very sensitive to DNA damaging agents. A single-copy suppressor screening to seek for a factor(s) that could rescue the drug sensitivity of sgs1Δmus81Δ100N cells was performed and revealed that Flp1, a site-specific recombinase 1 encoded on the 2-micron plasmid was a suppressor. Moreover, Flp1 overexpression could partially suppress the drug sensitivity of mus81Δ cells at 37 °C. Our findings suggest a possible function of Flp1 in coordination with Mus81 and Sgs1 to jointly resolve the branched-DNA structures generated in cells attempting to repair DNA damages.
机译：酿酒酵母Mus81是一种结构选择性核酸内切酶，与解旋酶-拓扑异构酶Sgs1-Top3-Rmi1复合物平行构成替代途径，以在DNA复制，修复和同源重组过程中解析许多DNA中间体。以前的研究表明，Mus81的N端区域是Sgs1冗余功能的体内功能所必需的;在N端缺少前120个氨基酸残基的mus81 Δ120N突变体与SGS1的丧失相结合，表现出合成的致死性。在这项研究中，进一步研究了Mus81 N末端区域在处理有毒中间体中的生理重要作用。我们检查了sgs1Δmus81Δ100N细胞的细胞缺陷，并观察到尽管这些细胞可行，但它们对DNA损伤剂非常敏感。进行了单拷贝抑制因子筛选，以寻找可以挽救sgs1Δmus81Δ100N细胞药物敏感性的因素，并揭示了Flp1，一种在2微米上编码的位点特异性重组酶1。质粒是抑制剂。此外，Flp1过表达可以部分抑制mus81Δ细胞在37°C时的药物敏感性。我们的发现表明Flp1与Mus81和Sgs1协同作用，可能共同解决试图修复DNA损伤的细胞中产生的支链DNA结构的可能功能。

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