Molecular characterization of the emnetrin-1 UNC-5/em receptor in emLucilia sericata/em larvae

Tahereh Karamzadeh; Hamzeh Alipour; Marziae Shahriari-Namadi; Abbasali Raz; Kourosh Azizi; Masoumeh Bagheri; Mohammad D. Moemenbellah-Fard

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Molecular characterization of the emnetrin-1 UNC-5/em receptor in emLucilia sericata/em larvae

机译：丝Luc幼虫 netrin-1 UNC-5 受体的分子特征

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Larval therapy with Lucilia sericata is a promising strategy in wound healing. Axon guidance molecules play vital roles during the development of the nervous system and also regulate the capacity of neuronal restoration in wound healing. Netrin-1 , one of the proteins that larvae secrete, plays a useful role in cell migration and nerve tissue regeneration. The UNC-5 receptor combines with a netrin-1 signal and transmits the signal from one side of the membrane to the other side, initiating a change in cell activity. In the current study, we identified the full length of the UNC-5 receptor mRNA in L. sericata using different sets of primers, including exon junction and specific region primers. The coding sequence (CDS) of the UNC-5 receptor was sequenced and identified to include 633 base-pair nucleic acids, and BLAST analysis on its nucleotide sequence revealed 96% identity with the Lucilia cuprina netrin-1 UNC-5 receptor . The protein residue included 210 amino acids (aa) and coded for a protein with 24 kD weight. This gene lacked the signal peptide. Furthermore, the UPA domain is conserved in UNC-5 . It lied at the interval of 26–131 aa. We identified the CDS of netrin-1 UNC-5 receptor in L. sericata . It could be applied to research activities implementing a new essential component design in wound healing. 展开▼

机译：Lucilia sericata的幼虫治疗是伤口愈合中有希望的策略。轴突导向分子在神经系统发育过程中起着至关重要的作用，并且还调节伤口愈合过程中神经元修复的能力。 Netrin-1是幼虫分泌的蛋白质之一，在细胞迁移和神经组织再生中起着有用的作用。 UNC-5受体与netrin-1信号结合，并将信号从膜的一侧传输到另一侧，从而启动细胞活性的变化。在当前的研究中，我们使用不同的引物集（包括外显子连接点和特定区域引物）鉴定了绢丝线虫中UNC-5受体mRNA的全长。对UNC-5受体的编码序列（CDS）进行测序并鉴定为包括633个碱基对核酸，并且对其核苷酸序列的BLAST分析显示与Lucilia cuprina netrin-1 UNC-5受体96％的同一性。蛋白质残基包含210个氨基酸（aa），并编码为重量为24 kD的蛋白质。该基因缺少信号肽。此外，UPA域在UNC-5中是保守的。它以26–131 aa的间隔撒谎。我们在丝线莲中鉴定了netrin-1 UNC-5受体的CDS。它可以应用于在伤口愈合中实施新的基本组件设计的研究活动。展开▼

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《AIMS Genetics》 |2019年第3期|共9页

作者
Tahereh Karamzadeh; Hamzeh Alipour; Marziae Shahriari-Namadi; Abbasali Raz; Kourosh Azizi; Masoumeh Bagheri; Mohammad D. Moemenbellah-Fard;
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中图分类遗传学;

关键词
molecular profileNetrin-1 UNC-5 receptorLucilia sericatablowfly larvae;

机译：分子概况Netrin-1 UNC-5受体Lucilia sericatablowfly幼虫;

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Molecular characterization of the emnetrin-1 UNC-5/em receptor in emLucilia sericata/em larvae

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