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首页> 外文期刊>AIMS Genetics >Relation between DNA damage measured by comet assay and OGG1 Ser326Cys polymorphism in antineoplastic drugs biomonitoring
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Relation between DNA damage measured by comet assay and OGG1 Ser326Cys polymorphism in antineoplastic drugs biomonitoring

机译：彗星试验检测的DNA损伤与抗肿瘤药物生物监测中的 OGG1 Ser326Cys多态性之间的关系

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Antineoplastic drugs are hazardous chemical agents used mostly in the treatment of patients with cancer, however health professionals that handle and administer these drugs can become exposed and develop DNA damage. Comet assay is a standard method for assessing DNA damage in human biomonitoring and, combined with formamidopyrimidine DNA glycosylase (FPG) enzyme, it specifically detects DNA oxidative damage. The aim of this study was to investigate genotoxic effects in workers occupationally exposed to cytostatics ( n = 46), as compared to a control group with no exposure ( n = 46) at two Portuguese hospitals, by means of the alkaline comet assay. The potential of the OGG1 Ser326Cys polymorphism as a susceptibility biomarker was also investigated. Exposure was evaluated by investigating the contamination of surfaces and genotoxic assessment was done by alkaline comet assay in peripheral blood lymphocytes. OGG1 Ser326Cys (rs1052133) polymorphism was studied by Real Time PCR. As for exposure assessment, there were 121 (37%) positive samples out of a total of 327 samples analysed from both hospitals. No statistically significant differences (Mann-Whitney test, p > 0.05) were found between subjects with and without exposure, regarding DNA damage and oxidative DNA damage, nevertheless the exposed group exhibited higher values. Moreover, there was no consistent trend regarding the variation of both biomarkers as assessed by comet assay with OGG1 polymorphism. Our study was not statistically significant regarding occupational exposure to antineoplastic drugs and genetic damage assessed by comet assay. However, health professionals should be monitored for risk behaviour, in order to ensure that safety measures are applied and protection devices are used correctly.
机译：抗肿瘤药物是主要用于治疗癌症患者的危险化学制剂，但是处理和管理这些药物的卫生专业人员可能会暴露在外并发展DNA损伤。彗星测定法是评估人类生物监测中DNA损伤的标准方法，并且与甲酰嘧啶DNA糖基化酶（FPG）酶结合使用，可专门检测DNA氧化损伤。这项研究的目的是通过碱性彗星试验研究与两家葡萄牙医院中未接触过细胞抑制剂（n = 46）的对照组相比，职业接触细胞抑制剂的工人的遗传毒性作用。还研究了OGG1 Ser326Cys多态性作为易感生物标志物的潜力。通过调查表面的污染程度评估暴露程度，并通过碱性彗星试验对外周血淋巴细胞进行遗传毒性评估。通过实时PCR研究了OGG1 Ser326Cys（rs1052133）多态性。至于接触评估，在两家医院分析的总共327份样本中，有121份（37％）阳性样本。在有和没有接触的受试者之间，在DNA损伤和氧化性DNA损伤方面没有发现统计学上的显着差异（Mann-Whitney检验，p> 0.05），尽管如此，接触组的值更高。此外，如通过OGG1多态性彗星试验评估的，两种生物标志物的变化均没有一致的趋势。我们的研究在职业性接触抗肿瘤药物和通过彗星试验评估的遗传损害方面没有统计学意义。但是，应监控健康专业人员的风险行为，以确保采取安全措施并正确使用保护装置。

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