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首页> 外文期刊>AIMS Agriculture and Food >A TaqMan PCR assay for detection of DGAT1 K232A polymorphism in cattle
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A TaqMan PCR assay for detection of DGAT1 K232A polymorphism in cattle

机译：TaqMan PCR检测牛中DGAT1 K232A多态性

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摘要

Metabolic processes involved in the control of fatty acid composition and ratio can greatly influence the quality of cow milk and beef. One of the key elements in this complex pathway is the enzyme diacylglycerol O-acyltransferase 1 (DGAT1). Numerous studies showed a strong correlation between DGAT1 K232A allelic variants and the content of saturated fat in cow milk and the level of beef marbling. PCR-RFLP, the widely used method for SNP detection, is laborious and time-consuming. This article describes the development of an efficient and rapid assay for detection of DGAT1 K232A polymorphism, based on real-time PCR with allele-specific TaqMan probes. The proposed method was validated by PCR-RFLP and the results fully coincided. Thus, our developed assay is efficient and reliable for rapid identification of DGAT1 K and A allelic variants in cattle that can be successfully applied in cattle breeding.
机译：控制脂肪酸组成和比例的代谢过程会极大地影响牛奶和牛肉的质量。此复杂途径中的关键要素之一是二酰基甘油O-酰基转移酶1（DGAT1）。大量研究表明，DGAT1 K232A等位基因变体与牛奶中饱和脂肪的含量和牛肉大理石花纹的含量之间具有很强的相关性。 PCR-RFLP是广泛用于SNP检测的方法，既费力又费时。本文介绍了基于等位基因特异性TaqMan探针的实时PCR技术，用于检测DGAT1 K232A多态性的快速有效测定方法的开发。通过PCR-RFLP验证了所提出的方法，结果完全吻合。因此，我们开发的测定方法对于快速鉴定牛中的DGAT1 K和A等位基因变异是有效而可靠的，可以成功地应用于牛育种。

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