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首页> 外文期刊>American Journal of Agriculture and Forestry >FISH Analysis of Oryza latifolia and Oryza alta Genomes with 45S rDNA Probes
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FISH Analysis of Oryza latifolia and Oryza alta Genomes with 45S rDNA Probes

机译：45S rDNA探针对稻米和水稻基因组的FISH分析

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摘要

Using Fluorescence in Situ Hybridization with 45S rDNA probes, we analyzed two wild rice species, Oryza alta and Oryza latifolia, both having CCDD genomes. The results showed that the hybridization signals of 45S rDNA in O.latifolia chromosome preparations were distributed on different chromosomes with the number of 10～16. The same signals in O.alta chromosome preparations were 6 and distributed on three pairs of homologous chromosomes: with two pairs in short chromosome arms and the other one pair in long chromosome arms. The number and positions of 45S rDNA signals are stable in O.alta genome, but there are some dynamic changes of signals in O.latifolia genome. Our observations indicated that there are distinct differences between the two CCDD genomes. Comparative analysis of the karyotype of O.alta and O.latifolia chromosomes based on the Fluorescence in Situ Hybridization pictures showed vast differences. We propose that the genome of O.alta may be differentiated earlier in evolution and tend to be stable, while O.latifolia may still be in its evolutionary process. Based on the imbalance of evolution and differences of genomic structure, we propose that O.alta and O.latifolia can be divided into two wild rice species, which may be better conforming with their evolution characteristics. The mechanism and characteristics of 45s rDNA's distribution in the chromosomes are discussed.
机译：使用与45S rDNA探针进行的荧光原位杂交，我们分析了两个野生稻种Oryza alta和Layfolia，它们都具有CCDD基因组。结果表明，45S rDNA杂交信号在O.latifolia染色体制备物中分布在不同的染色体上，数目为10〜16。 O.alta染色体制剂中的相同信号为6，分布在三对同源染色体上：两对在短染色体臂中，另一对在长染色体臂中。 45S rDNA信号的数目和位置在O.alta基因组中是稳定的，但是在O.latifolia基因组中信号有一些动态变化。我们的观察结果表明，两个CCDD基因组之间存在明显差异。基于荧光原位杂交图像的O.alta和O.latifolia染色体核型的比较分析显示出巨大的差异。我们建议O.alta的基因组可能在进化中较早分化并趋于稳定，而O.latifolia可能仍处于其进化过程中。基于进化的不平衡和基因组结构的差异，我们建议将O.alta和O.latifolia分为两种野生稻，这可能更符合它们的进化特征。探讨了45s rDNA在染色体中分布的机理和特征。

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