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首页> 外文期刊>American journal of agricultural and biological sciences >Comparison of Molecular Markers ISSR, SSR and RFLP to Study the Genetic Variation of Strawberry Cultivars
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Comparison of Molecular Markers ISSR, SSR and RFLP to Study the Genetic Variation of Strawberry Cultivars

机译：分子标记ISSR，SSR和RFLP的比较研究草莓品种的遗传变异

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摘要

Strawberry is grown in Indonesia because it has potential high economic value. The purpose of this study was to investigate relationship of phenetic lineage and genetic variations in 12 strawberry cultivars using three different molecular marker methods, including ISSR, SSR and RFLP. Five primers of ISSR markers were used and they generated 53 bands with 44 polymorphic DNA bands. Percentage of polymorphic using ISSR method was 83%. Five primers of SSR markers were used and they gave 41 DNA bands with 36 polymorphic DNA bands. Percentage of polymorphic using SSR method was 87%. Four primers of PCR-RFLP markers and 3 enzymes were used and they had 20 DNA bands with 9 polymorphic DNA bands. Percentage of polymorphic PCR-RFLP method was 45%. Three dendrogram results based on each method indicated that Stroberi Hitam has smallest similarity index compared with other eleven cultivars. 'Californica' cultivar had the closest similarity with Stroberi Hitam. Data analysis of molecular character requires complementary and supporting data of other characters such as morphology, biochemistry and cytology so this will strengthen the lineage data of the organisms to be investigated. Molecular marker can be used as a good method for genetic variation analysis if it has some advantages such as high polymorphism, codominance and specific characteristics and wide distribution.
机译：草莓在印度尼西亚种植，因为它具有很高的经济价值。本研究的目的是使用ISSR，SSR和RFLP等三种不同的分子标记方法研究12个草莓品种的物系和遗传变异之间的关系。使用了5个ISSR标记引物，它们产生了53条带，具有44条多态性DNA条带。使用ISSR方法的多态性百分比为83％。使用了5个SSR标记的引物，它们给出了41条DNA条带和36条多态性DNA条带。使用SSR方法的多态性百分比为87％。使用了4条PCR-RFLP标记引物和3种酶，它们具有20条DNA条带和9条多态性DNA条带。多态PCR-RFLP方法的百分比为45％。基于每种方法的三个树状图结果表明，与其他11个品种相比，Stroberi Hitam具有最小的相似性指数。 “加州”品种与斯特罗贝里·希淡最相似。分子特征的数据分析需要其他特征（例如形态，生物化学和细胞学）的补充和支持数据，因此这将加强要研究的生物的谱系数据。分子标记具有多态性强，共性强，特异性强，分布广泛等优点，可以作为遗传变异分析的一种很好的方法。

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