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首页> 外文期刊>American Journal of Analytical Chemistry >Determination of Rebaudioside A and Stevioside in Leaves of S. rebaudiana Bertoni Grown in México by a Validated HPLC Method
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Determination of Rebaudioside A and Stevioside in Leaves of S. rebaudiana Bertoni Grown in México by a Validated HPLC Method

机译：经验证的HPLC方法测定墨西哥种植的雷波氏酵母柏叶中的莱鲍迪甙A和甜菊甙

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Stevia rebaudiana is a plant with high sweetening capacity due to its content of glycosides, mainly stevioside and rebaudioside A. Several techniques have been used to determine the concentrations of glycosides in Stevia, although an HPLC method is recommended by the FAO/WHO-JECFA. Varieties of Stevia have been recently grown in Mexico, with no previous report of glycosides by a validated method. The aim of this study was to validate an isocratic HPLC method for content determination of main glycosides in the leaves of Stevia cultivated in Mexico. HPLC method was performed using a C18 column (250 mm × 4.6 mm, 5 μm) and UV detector set at 210 nm. The mobile phase consisted of 32:68 (v/v) mixture of acetonitrile and sodium-phosphate buffer (10 mmol/L, pH 2.6), set to a flow rate of 1.0 mL/min. Rebaudioside A and stevioside were determined in two Stevia varieties: Morita II and Criolla, and also validation parameters were calculated. Rebaudioside A content (g/100g) in Morita II was 15.15 ± 0.02 while stevioside was 3.97 ± 0.003; in the case of Criolla they were 4.03 ± 0.01 and 8.80 ± 0.14, respectively (p < 0.001). The recoveries of fortified samples were 100% ± 10% and precision RSD was ≤6.27%. The criteria of validation showed accuracy, linearity (≥0.99), and precision; therefore, the determination of glycosides was performed with reliability.
机译：甜叶菊甜叶菊由于其糖苷含量较高（主要是甜菊糖苷和莱鲍迪苷A），具有很高的增甜能力。尽管粮农组织/世界卫生组织-JECFA建议采用HPLC方法，但已使用多种技术确定甜叶菊中糖苷的浓度。甜叶菊的品种最近在墨西哥种植，以前没有通过有效方法对糖苷的报道。这项研究的目的是验证一种等度HPLC方法，用于测定在墨西哥种植的甜叶菊叶片中主要糖苷的含量。 HPLC方法使用C18色谱柱（250 mm×4.6 mm，5μm）和设置在210 nm的UV检测器进行。流动相由乙腈和磷酸钠缓冲液（10 mmol / L，pH 2.6）的32:68（v / v）混合物组成，流速设置为1.0 mL / min。在两个甜叶菊变种中测定了莱鲍迪甙A和甜菊糖甙：森田II和克里奥拉，并且还计算了验证参数。森田II中的莱鲍迪甙A含量（g / 100g）为15.15±0.02，甜菊糖甙为3.97±0.003;对于Criolla，它们分别为4.03±0.01和8.80±0.14（p <0.001）。强化样品的回收率为100％±10％，精密RSD≤6.27％。验证标准显示出准确性，线性（≥0.99）和准确性;因此，糖苷的测定是可靠的。

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