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首页> 外文期刊>American Journal of Analytical Chemistry >Qualitative and Quantitative Analysis of Five Bioactive Flavonoids in Salix bordensis Turcz. by HPLC-DAD and HPLC-ESI-MS
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Qualitative and Quantitative Analysis of Five Bioactive Flavonoids in Salix bordensis Turcz. by HPLC-DAD and HPLC-ESI-MS

机译：紫柳中五种生物活性黄酮的定性和定量分析。 HPLC-DAD和HPLC-ESI-MS分析

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摘要

The qualitative characterization and quantitative analysis of five bioactive flavonoids in Salix bordensis Turcz. were achieved via reversed-phase high-performance liquid chromatography coupled with diode array detection and tandem mass spectrometry, by using an Agilent ZORBAX SB-C18 HPLC column with a gradient elution of 0.3% (v/v) formic acid in water and methanol as the mobile phase. The compounds in the mixture were clearly identified by comparing their HPLC-DAD ultraviolet spectra, retention times, and MS data with those of corresponding reference compounds. All calibration curves showed good linearity (r2 > 0.9998) within the test ranges. The LOD, LOQ, specificity, precision, and accuracy for the method were validated. The results demonstrated that this analytical approach is ideal for the determination of bioactive compounds, such as flavonoids, and that it constructed a basis for the comprehensive evaluation of the quality of Salix bordensis Turcz.
机译：紫柳中5种生物活性黄酮的定性表征和定量分析。通过使用配备有0.3％（v / v）甲酸在水和甲醇中的梯度洗脱的安捷伦ZORBAX SB-C18 HPLC色谱柱，通过反相高效液相色谱结合二极管阵列检测和串联质谱来实现作为流动相。通过将其HPLC-DAD紫外光谱，保留时间和MS数据与相应的参考化合物进行比较，可以清楚地识别混合物中的化合物。在测试范围内，所有校准曲线均显示出良好的线性（r2> 0.9998）。验证了该方法的LOD，LOQ，特异性，精密度和准确性。结果表明，该分析方法是测定生物活性化合物（如黄酮类化合物）的理想方法，并且为综合评价柳柳质量提供了基础。

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