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首页> 外文期刊>American Journal of Drug Discovery and Development >Antioxidant and Antimicrobial Activities of the Volatile Oil of Ocimum gratissimum and its Inhibition on Partially Purified and Characterized Extracellular Protease of Salmonella enteritidis
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Antioxidant and Antimicrobial Activities of the Volatile Oil of Ocimum gratissimum and its Inhibition on Partially Purified and Characterized Extracellular Protease of Salmonella enteritidis

机译：香茅挥发油的抗氧化和抑菌活性及其对肠炎沙门氏菌部分纯化和表征的细胞外蛋白酶的抑制作用

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The emergence of antibiotic resistant bacteria has made the treatments of pathogenic infections difficult. The discovery that some analytes of plant extracts are active against multi-drug resistant bacteria has opened ways to researching into the antimicrobial activity of these phytocomponents. Studies are designed to determine the antimicrobial and antioxidant potentials of the volatile oil of Ocimum gratissimum and its inhibition on partially purified and characterized extracellular protease of Salmonella enteritidis. The oil was extracted by hydrodistillation. Its antimicrobial effect was carried out using microdilution method while antioxidant effect was tested against 1,1-diphenyl-2-picrylhydrazyl (DPPH). The caseinolytic activity of the enzyme was studied with oil as inhibitor. The enzyme was partially purified with dialysis and gel filtration. The oil has scavenging activity of 66.98±1.78% compared to butylated hydroxyltoluene (positive control), 53.29±2.51% (sig. p<0.0001) against DPPH. Salmonella enteritidis was inhibited by this oil with IC50 of 3.98% (v/v). The enzyme had optimal activities at 45°C and pH 7.5. None of the metallic chloride tested produced any significant increase in the activity of the enzyme. Hg2+ and Pb2+ were inhibitors of this enzyme. The oil showed a non-competitive inhibition with Km = 0.33 mg mL-1, Vmax = 1.25x103 μmol min-1 (oil absent) and V’max = 2.50x102 μmol min-1 (oil present). The highest purification fold = 1.88 and the highest percentage yield = 51.74 as compared to the crude enzyme extract. Therefore, the volatile oil of Ocimum gratissimum possessed antimicrobial and antioxidant activities, its inhibition against this protease may be one of the ways of its antimicrobial effect.
机译：抗生素抗性细菌的出现使得病原体感染的治疗变得困难。植物提取物的某些分析物对多药耐药细菌具有活性的发现为研究这些植物成分的抗微生物活性开辟了途径。设计研究以确定释放性罗汉果挥发油的抗微生物和抗氧化能力及其对肠炎沙门氏菌部分纯化和表征的细胞外蛋白酶的抑制作用。通过加氢蒸馏提取油。其抗微生物作用是通过微量稀释法进行的，而抗氧化作用则针对1,1-二苯基-2-吡啶并肼基（DPPH）进行了测试。以油为抑制剂研究了酶的酪蛋白分解活性。通过透析和凝胶过滤将酶部分纯化。与丁基化羟基甲苯（阳性对照），53.29±2.51％（sig。p <0.0001）相比，该油具有66.98±1.78％的清除活性。该油抑制肠炎沙门氏菌，IC50为3.98％（v / v）。该酶在45℃和pH 7.5下具有最佳活性。所测试的金属氯化物均未产生酶活性的任何显着增加。 Hg2 +和Pb2 +是该酶的抑制剂。该油显示出非竞争性抑制作用，Km = 0.33 mg mL-1，Vmax = 1.25x103μmolmin-1（无油），V'max = 2.50x102μmolmin-1（有油）。与粗酶提取物相比，最高的纯化倍数＝ 1.88，最高的百分产率＝ 51.74。因此，无花果挥发油具有抗微生物和抗氧化的活性，其对这种蛋白酶的抑制作用可能是其抗微生物作用的途径之一。

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