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首页> 外文期刊>American journal of food technology >Effect of Cellulases, Solvent Type and Particle Size Distribution on the Extraction of Chlorogenic Acid and Other Phenols from Spent Coffee Grounds
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Effect of Cellulases, Solvent Type and Particle Size Distribution on the Extraction of Chlorogenic Acid and Other Phenols from Spent Coffee Grounds

机译：纤维素酶，溶剂类型和粒度分布对废咖啡渣中绿原酸和其他酚的提取的影响

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Spent coffee grounds, wastes resulting from the industrial preparation of instant coffee, were subjected to solid-liquid extractions to study the influence of some critical variables on the phenol content of extracts. After grinding, spent coffee grounds were passed through several sieve sizes (125, 250, 500 and 1000 μm) and classified into four different particle size groups. The highest yields of total phenols were consistently obtained from the smallest particles and an unexpected reduction in the phenol release was observed when extraction was assisted by cellulase treatment. Aqueous ethanol (60% w/w) was the solvent having the highest phenol-extractive capacity, closely followed by aqueous methanol, whose extracts were ~30% more concentrated than when pure water was used as the solvent. Phenol concentration values obtained from the different treatments ranged from 115-400 mg equivalents of chlorogenic acid/l. HPLC analysis confirmed chlorogenic acid as the major phenolic acid being extracted from spent coffee grounds. Chromatograms of extracts obtained after the enzyme treatment showed that cellulases catalyzed the transformation of chlorogenic acid, resulting in a derivative with similar spectrum, but shorter retention time. Results shown in this study are a first step for further research on the extractive conditions maximizing extraction efficiency from spent coffee grounds, confirming the feasibility of upgrading spent coffee grounds as a promising source of chlorogenic acid, which may be used in biofunctional dietary supplements.
机译：用过的咖啡渣，即速溶咖啡的工业制备产生的废物，经过固液萃取，以研究一些关键变量对萃取物中酚含量的影响。磨碎后，将用过的咖啡渣通过几种筛尺寸（125、250、500和1000μm），并分为四个不同的粒径组。从最小的颗粒中始终可以得到最高的总酚含量，当通过纤维素酶处理辅助提取时，可以观察到苯酚释放的意外减少。乙醇水溶液（60％w / w）是具有最高苯酚提取能力的溶剂，紧随其后的是甲醇水溶液，其提取物的浓度比使用纯水作为溶剂时高约30％。通过不同处理获得的苯酚浓度值范围为115-400 mg当量的绿原酸/ l。 HPLC分析证实绿原酸是从废咖啡渣中提取的主要酚酸。酶处理后获得的提取物的色谱图表明，纤维素酶催化了绿原酸的转化，从而产生了具有相似光谱但保留时间更短的衍生物。这项研究显示的结果是进一步研究可最大程度地从废咖啡渣中提取效率的提取条件的第一步，证实了将废咖啡渣升级为有希望的绿原酸来源的可行性，可将其用于生物功能性膳食补充剂中。

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