...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Microbiology >PHENOTYPICAL AND MASS SPECTRAL ASSESSMENT METHODS FOR IDENTIFICATION OF SOME CONTAGIOUS MASTITIS PATHOGENS | Science Publications
【24h】

PHENOTYPICAL AND MASS SPECTRAL ASSESSMENT METHODS FOR IDENTIFICATION OF SOME CONTAGIOUS MASTITIS PATHOGENS | Science Publications

机译：鉴定某些传染性乳突病的表型和质谱分析方法科学出版物

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

> Mastitis is one of the most economic disease affecting dairy cows worldwide. Identification of mastitis pathogens still depends principally on culture and phenotypical method, which is a difficult and time-consuming. Newly, microbiologists have focused their attention on the use of Mass Spectrometry (MS) for microbial identification, especially Matrix Assisted Laser Desorption Ionization Time-Of-Flight (MALDI-TOF). Therefore, this study was designated to evaluate the ability of MALDI-TOF to identify some contagious mastitis pathogens comparing with phenotypical methods such as API panels and VITEK 2 system. A total of one hundred twenty of Staphylococcus aureus (S. aureus), Coagulase Negative Staphylococci (CNS) and Streptococcus agalactiae (Strept. agalactiae) strains isolated from milk of cows affected by clinical and subclinical mastitis were used in the study. According to the results, ~95% of S. aureus, 100% of CNS and Strept. agalactiae were correctly identified by MALDI TOF MS. All S. aureus isolates were then confirmed by a nuc-based PCR technique. While ~92% of S. aureus, 87% of Strept. agalactiae and 76% of CNS were identified by VITEK 2 system. Moreover, ~89% of S. aureus, 80% of Strept. agalactiae and 72% of CNS were identified by API system. In brief, the results demonstrated that MALDI-TOF is a fast and truthful technique which has the capability to replace conventional identification of several bacterial strains usually isolated in clinical laboratories of microbiology. Therefore, it is recomended that MALDI-TOF MS technology can be regularly used in veterinary laboratories for identification of different species of bacteria, particularly when failure of phenotypic methods forces clinical microbiologists.
机译： >乳腺炎是影响全世界奶牛的最经济的疾病之一。乳腺炎病原体的鉴定仍然主要取决于培养和表型方法，这是困难且耗时的。最近，微生物学家将注意力集中在质谱（MS）用于微生物鉴定上，尤其是基质辅助激光解吸电离飞行时间（MALDI-TOF）。因此，本研究旨在评估MALDI-TOF与表型方法（如API面板和VITEK 2系统）相比，识别某些传染性乳腺炎病原体的能力。总共一百二十个金黄色葡萄球菌（ S。aureus ），凝固酶阴性葡萄球菌（CNS）和无乳链球菌（链球菌该研究使用了从受临床和亚临床乳腺炎影响的母牛的牛奶中分离出的无乳杆菌菌株。根据结果​​，约95％的S。金黄色葡萄球菌，100％的CNS和 Strept。 MALDI TOF MS正确鉴定了无乳杆菌。所有 S。然后通过基于nuc的PCR技术确认金黄色葡萄球菌分离株。而约92％的硫。金黄色，占链的87％。通过VITEK 2系统鉴定出无乳杆菌和76％的中枢神经系统。此外，约89％的硫。金黄色葡萄球菌，占链霉菌的80％。通过API系统鉴定出无乳杆菌和72％的中枢神经系统。简而言之，结果表明，MALDI-TOF是一种快速而真实的技术，能够代替通常在微生物学临床实验室中分离的几种细菌菌株的常规鉴定。因此，建议在兽医实验室中定期使用MALDI-TOF MS技术来鉴定不同种类的细菌，尤其是当表型方法的失败迫使临床微生物学家使用时。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号