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首页> 外文期刊>American Journal of Microbiology >TESTING CARBOXYMETHYL CELLULASE ACTIVITY SECRETED BY TRICHOPHYTON TERRESTRE IN CARBOXYMETHYL CELLULOSE SOLUTION | Science Publications
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TESTING CARBOXYMETHYL CELLULASE ACTIVITY SECRETED BY TRICHOPHYTON TERRESTRE IN CARBOXYMETHYL CELLULOSE SOLUTION | Science Publications

机译：在羧甲基纤维素溶液中检测毛滴虫地形产生的羧甲基纤维素酶活性科学出版物

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摘要

> The techniques employed for bioconversion of cellulosic biomass to simple sugars by cellulases have a great industrial brunt. Cold-adapted microorganisms are potential resource of cold-active carboxymethyl cellulases secluded from the cold regions. In this study, Trichophyton terrestre-a rare fungal species in Indian soils, isolated from the rhizosphere of Juglans regia L. during winter season was subjugated for the production and commotion of an enzyme carboxymethyl cellulase in carboxymethyl cellulose solution by DNS method at an ample range of temperatures, using Lineweaver-Burk plot which offers a practical graphical method for the analysis of Michealis-Menten equation, to establish the imperative terms in enzyme kinetics as Km and Vmax. The enzyme kinetic parameters like maximum activity (Vmax), Km and turnover number were recorded at varying concentrations of CMC and different temperatures (4?°C and 50?°C). The enzyme was found to be tolerant and stable at two varying temperatures which enables this fungal species to survive in the extreme environmental conditions of northern India. Such property of carboxymethyl cellulase enzyme has extensive range of applications and the potential to open new application fields in the areas of industrial processes.
机译： >通过纤维素酶将纤维素生物质生物转化为单糖的技术首当其冲。适应寒冷的微生物是从寒冷地区隔离出来的冷活性羧甲基纤维素酶的潜在资源。在这项研究中，T.richophyton terrestre（一种印度土壤中的稀有真菌物种，在冬季从Juglans regia L.的根际中分离出来）被用于生产和迁移。 DNS方法在足够的温度范围内，通过酶法在羧甲基纤维素溶液中制备羧甲基纤维素酶> m 和V max 。在不同的CMC浓度和不同温度（4℃和50℃）下记录酶动力学参数，如最大活性（V max ），K m 和周转数。 C）。发现该酶在两个不同的温度下均具有耐受性和稳定性，使该真菌物种能够在印度北部的极端环境条件下生存。羧甲基纤维素酶的这种特性具有广泛的应用范围，并有可能在工业过程领域中开拓新的应用领域。

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