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Free radical generation by skeletal muscle of adult and old mice: effect of contractile activity

机译：成年和老年小鼠骨骼肌自由基生成：收缩活动的影响

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Oxidative modification of cellular components may contribute to tissue dysfunction during aging. In skeletal muscle, contractile activity increases the generation of reactive oxygen and nitrogen species (ROS). The question of whether contraction-induced ROS generation is further increased in skeletal muscle of the elderly is important since this influences recommendations on their exercise participation. Three different approaches were used to examine whether aging influences contraction-induced ROS generation. Hind limb muscles of adult and old mice underwent a 15-min period of isometric contractions and we examined ROS generation by isolated skeletal muscle mitochondria, ROS release into the muscle extracellular fluid using microdialysis techniques, and the muscle glutathione and protein thiol contents. Resting skeletal muscle of old mice compared with adult mice showed increased ROS release from isolated mitochondria, but no changes in the extracellular levels of superoxide, nitric oxide, hydrogen peroxide, hydroxyl radical activity or muscle glutathione and protein thiol contents. Skeletal muscle mitochondria isolated from both adult and old mice after contractile activity showed significant increases in hydrogen peroxide release compared with pre-contraction values. Contractions increased extracellular hydroxyl radical activity in adult and old mice, but had no significant effect on extracellular hydrogen peroxide or nitric oxide in either group. In adult mice only, contractile activity increased the skeletal muscle release of superoxide. A similar decrease in muscle glutathione and protein thiol contents was seen in adult and old mice following contractions. Thus, contractile activity increased skeletal muscle ROS generation in both adult and old mice with no evidence for an age-related exacerbation of ROS generation.
机译：细胞成分的氧化修饰可能会导致衰老过程中的组织功能障碍。在骨骼肌中，收缩活动会增加活性氧和氮物质（ROS）的生成。收缩引起的ROS的产生是否在老年人的骨骼肌中进一步增加的问题很重要，因为这会影响对他们的运动参与的建议。使用三种不同的方法来检查老化是否影响收缩诱导的ROS生成。成年和成年小鼠的后肢肌肉进行等距收缩15分钟，我们检查了孤立骨骼肌线粒体产生的ROS，使用微透析技术释放到肌肉细胞外液中的ROS，以及肌肉中的谷胱甘肽和蛋白质硫醇含量。与成年小鼠相比，老年小鼠的静息骨骼肌显示出从分离的线粒体中释放的ROS增加，但细胞外超氧化物，一氧化氮，过氧化氢，羟基自由基活性或肌肉中谷胱甘肽和蛋白质硫醇含量没有变化。与收缩前的值相比，收缩活动后从成年和老年小鼠中分离出的骨骼肌线粒体显示过氧化氢释放量显着增加。收缩增加成年和成年小鼠的细胞外羟基自由基活性，但对两组中的细胞外过氧化氢或一氧化氮均无显着影响。仅在成年小鼠中，收缩活动增加了骨骼肌超氧化物的释放。收缩后，成年和成年小鼠的肌肉中谷胱甘肽和蛋白质硫醇含量也有类似的下降。因此，收缩活动增加了成年和成年小鼠的骨骼肌ROS生成，没有证据表明与年龄相关的ROS生成加剧。

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