• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Epidemiology and Infectious Disease >Genotyping and Phylogenetic Analysis of Cystic Echinococcosis Isolated from Camels and Humans in Egypt
【24h】

Genotyping and Phylogenetic Analysis of Cystic Echinococcosis Isolated from Camels and Humans in Egypt

机译：埃及骆驼和人类分离的囊性棘球cc虫病的基因分型和系统发育分析

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The objectives of the present study were to investigate strain identification of Echinococcus granulosus infecting camel and human in . Therefore partial sequences were generated after gel purification of nested PCR amplified products of mitochondrial NADH 1gene of Echinococcus granulosus complex. Sequences were further examined by sequence analysis and subsequent phylogeny to compare these sequences to those from known strains of E.granulosus circulating globally and retrieved from GenBank. All isolates are homologous to the camel strain, E. canadensis (G6) genotype. Nucleotide mutations generate polymorphism at position of 275 nucleotide, where a thymine replaced a cytosine and at the levels of 385 and 386 nucleotides, where two cytosine substituted a guanine and a thymine respectively. KF815488 Egypt showed typical identity (99.5%) with JN637176 Sudan, HM853659 Iran, AF386533 France and AJ237637 Poland with 0.5% diversion.. Phylogenetic analysis showed a robust tree clustering all isolates with sequences belonging to the camel genotype (G6) variant with strong bootstrap values at relevant nodes and the evolutionary distance between groups is very short. There are two mutations in the sequences of amino acids at the position of 92, where an Alanine is changed to a Valine and at the position of 129, where a Valine is transformed to a Proline. Our record of a single genotype determined a strain which could be incriminated for camel and human infectivity and responsible for its persistence in the endemic areas. Such epidemiological data could guide the application of efficient control strategies of hydatidosis in Egypt.
机译：本研究的目的是调查感染骆驼和人的细粒棘球strain的菌株鉴定。因此，凝胶纯化细粒棘球E虫线粒体NADH 1基因的巢式PCR扩增产物后，产生了部分序列。通过序列分析和随后的系统发育进一步检查序列，以将这些序列与从全球流通并从GenBank检索到的已知的E.granulosus菌株的序列进行比较。所有分离物都与骆驼菌株加拿大E. canadensis（G6）基因型同源。核苷酸突变在275个核苷酸的位置上产生多态性，其中胸腺嘧啶取代了胞嘧啶，在385和386个核苷酸的水平上，其中两个胞嘧啶分别取代了鸟嘌呤和胸腺嘧啶。 KF815488埃及与JN637176苏丹，HM350659伊朗，AF386533法国和AJ237637波兰表现出典型的同一性（99.5％），转移率为0.5％。相关节点的值和组之间的进化距离非常短。氨基酸序列中有两个突变，其中第92位的位置是丙氨酸变为缬氨酸，第129位的位置是氨基酸转化为脯氨酸。我们对单一基因型的记录确定了一种可归因于骆驼和人类传染性的菌株，并归因于其在流行地区的持久性。这样的流行病学数据可以指导埃及的hy虫病有效控制策略的应用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号