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首页> 外文期刊>American Journal of Experimental Agriculture >Genetic Characterization of Alupe Napier Grass Accessions Based on Simple Sequence Repeat Markers
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Genetic Characterization of Alupe Napier Grass Accessions Based on Simple Sequence Repeat Markers

机译：基于简单序列重复标记的Alupe内皮草种质的遗传特征

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摘要

Genetic variability of 23 Napier grass clones, 22 of which were collected from Kenya Agricultural Research Institute – at Alupe in Kenya was determined using 17 simple sequence repeat (SSR) markers. All markers were polymorphic with the most discriminative being PSMP2267. Average gene diversity among the Napier grass clones was 0.54 with an average heterozygosity of 0.63. Total number of alleles across all loci was 90 with mean number of alleles per locus of 5.29 and a mean polymorphic information content of 0.50. Results also indicate a high genetic distance among the Napier grass clones with the most distant clone being 16814 while the closest was between kakamega1 and kakamega2 and kakamega2 and kakamega3. Principal Co-ordinates Analysis did not group the clones in a definitive structure, with most clones scattered. However, the SSR markers grouped the clones into two clusters derived from a dendogram generated by Neighbour joining analysis with clone 16814 being alone in a cluster. Clone Kakamega1, Kakamega2 and Kakamega3 as very closely related. These findings are key in guiding selection of clones for inclusion in breeding programs and conservation of Napier grass.
机译：使用17个简单序列重复（SSR）标记确定了23个纳皮尔草无性系的遗传变异性，其中22个是从肯尼亚农业研究所肯尼亚的Alupe收集的。所有标记都是多态性的，最有区别的是PSMP2267。纳皮尔草克隆的平均基因多样性为0.54，平均杂合度为0.63。所有基因座上的等位基因总数为90，每个基因座的平均等位基因数量为5.29，平均多态信息含量为0.50。结果还表明，纳皮尔草克隆之间的遗传距离较高，最远的克隆为16814，而最接近的克隆在kakamega1和kakamega2之间，以及kakamega2和kakamega3之间。首席坐标分析没有将克隆按确定的结构分组，大多数克隆是分散的。但是，SSR标记将克隆分为两个簇，两个簇均来自通过邻居加入分析生成的树状图，而克隆16814仅在簇中。克隆Kakamega1，Kakamega2和Kakamega3密切相关。这些发现是指导克隆选择纳入繁殖计划和保护纳皮尔草的关键。

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