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首页> 外文期刊>American Journal of Experimental Agriculture >Effect of Probiotic, Carbohydrase Enzyme and Their Combination on the Performance, Histomorphology and Gut Microbiota in Broilers Fed Wheat-based Diets
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Effect of Probiotic, Carbohydrase Enzyme and Their Combination on the Performance, Histomorphology and Gut Microbiota in Broilers Fed Wheat-based Diets

机译：益生菌，碳水化合物酶及其组合对饲喂小麦饮食的肉鸡生产性能，组织形态和肠道菌群的影响

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The effect of supplementation of probiotic and/or carbohydrase on performance and gut health in broiler chickens was studied. Diet 1 was the basal diet with no supplement while diets 2, 3, 4 and 5 were supplemented with antibiotic, probiotic, carbohydrase and combination of probiotic and carbohydrase, respectively in a randomized complete block design. The experiment was carried out at the Poultry Unit of the Teaching and Research Farm, University of Ibadan, Nigeria between March and April, 2013. 240 one-day-old broiler chickens were allotted to 5 diets with 6 replicates of 8 birds each in a 35-day experiment. On day 35, birds were sacrificed and digesta samples from the duodenum, ileum, caecum and colon were collected for microbial load count. Digesta viscosity and pH from the ileum were also determined. Sections of the ileum were obtained for morphological measurements. Result showed that diet had no effect on the feed intake, dry matter intake and feed conversion ratio of birds at starter (d 0-21), grower phases (d 22-35) and overall period (d 0-35). Body weight gain was significantly (P = .05) improved at d 0-21 and 22-35 with the addition of probiotic and/or carbohydrase but not at d 0-35. Diet had no effect on the microbiota in the various segments of the GIT of birds. Coliform counts of birds fed diet supplemented with carbohydrase were significantly (P = .05) higher (5.64 x 106 cfu/ml) than the values in other diets. The crypt depth, villus height, villus width and villus height:crypt depth ratio were not affected by diet. Highest digesta viscosity (1072mPa) was recorded in birds on the negative control while the least digesta viscosity (529mPa) was observed in birds on diet supplemented with carbohydrase enzyme. It is concluded that probiotic and/or carbohydrase are viable feed supplements in wheat-based diets for broilers.
机译：研究了补充益生菌和/或糖酶对肉鸡生产性能和肠道健康的影响。饮食1是基本饮食，无补充，而饮食2、3、4和5分别以随机完整块设计补充了抗生素，益生菌，糖酶以及益生菌和糖酶的组合。该实验于2013年3月至4月在尼日利亚伊巴丹大学教学与研究农场的家禽部门进行。将240只1日龄的肉鸡分配到5种日粮中，每组6只，每只重复8只鸡35天的实验。在第35天，处死鸟并收集来自十二指肠，回肠，盲肠和结肠的消化物样品以进行微生物负荷计数。还确定了回肠的消化粘度和pH。获得回肠的切片用于形态学测量。结果表明，饲喂日粮（d 0-21），成年期（d 22-35）和整个时期（d 0-35）对家禽的采食量，干物质采食量和饲料转化率没有影响。加入益生菌和/或糖酶在d 0-21和22-35时体重增加显着（P = 0.05），但在d 0-35时没有增加。饮食对禽类胃肠道各个部分的微生物没有影响。饲喂添加了碳水化合物的日粮的大肠菌群计数比其他日粮中的值显着高（P = 0.05）（5.64 x 106 cfu / ml）。隐窝深度，绒毛高度，绒毛宽度和绒毛高度：隐窝深度比不受饮食的影响。补充了碳水化合物酶的日粮中，鸟类的最高消化酶粘度（1072mPa）记录在禽中，而最低的消化物粘度（529mPa）在鸟类中观察到。结论是，益生菌和/或碳水化合物酶是用于肉鸡的小麦日粮中可行的饲料补充剂。

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