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首页> 外文期刊>American Journal of Experimental Agriculture >Germination and Development of 'Precocinho' Peach Embryos: Asepsis and Use of PPMTM in Culture Medium
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Germination and Development of 'Precocinho' Peach Embryos: Asepsis and Use of PPMTM in Culture Medium

机译：'Precocinho'桃胚的发芽和发育：无菌和培养基中PPMTM的使用

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摘要

Embryo culture is a technique used to rescue immature embryos which would not germinate under natural conditions. However, one of the biggest problems of this process is the contamination by microorganisms. The objective of this work was to test asepsis methods and the use of PPMTM (Plant Preservative MixtureTM) in the culture medium for the germination and development of 'Precocinho' peach embryos. After extraction from the fruits, seeds were submitted to treatments with thimerosal, sodium hypochlorite (NaClO), flame sterilization and moxifloxacin hydrochloride, in culture medium with and without addition of PPMTM, totaling nine treatments. The experimental design was completely randomized, with 20 replicates per treatment. Three evaluations were performed: in vitro establishment (at 55 days of cultivation), embryo development (at 65 days of cultivation) and acclimatization (20 days after transplanting in the greenhouse). Six treatments did not had any contamination, on the first evaluation. However, 65 days after culture, the NaClO with PPMTM addition presented the best results for the variables analyzed. The average seedling survival was 81.6%, after acclimatization. The use of sodium hypochlorite with the addition of PPMTM is shown as the most efficient treatment, among the tested ones, for the germination and development of 'Precocinho' peach embryos.
机译：胚胎培养是一种用于挽救在自然条件下不会发芽的未成熟胚的技术。然而，该过程的最大问题之一是微生物的污染。这项工作的目的是测试无菌方法以及在培养基中使用PPM TM （植物防腐剂混合物 TM ）来促进'Precocinho'桃的发芽和发育胚胎。从果实中提取后，将种子在不添加PPM TM 的培养基中进行硫柳汞，次氯酸钠（NaClO），火焰灭菌和盐酸莫西沙星的处理，共进行9种处理。实验设计完全随机化，每次处理重复20次。进行了三个评估：体外建立（在培养55天时），胚胎发育（在培养65天时）和驯化（在温室中移植后20天）。在第一次评估中，六种处理没有任何污染。然而，培养65天后，添加PPM TM 的NaClO对分析的变量表现出最好的结果。适应后，幼苗平均存活率为81.6％。在测试的蛋白质中，使用次氯酸钠和PPM TM 被证明是最有效的处理方法，用于“ Precocinho”桃子胚的萌发和发育。

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