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首页> 外文期刊>Annals of microbiology >Comparison of various culture methods (Skirrow medium, a blood-free medium and a filtration system enriched in Bolton and Preston broths) for isolation of Campylobacter spp. from raw meat samples
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Comparison of various culture methods (Skirrow medium, a blood-free medium and a filtration system enriched in Bolton and Preston broths) for isolation of Campylobacter spp. from raw meat samples

机译：比较用于分离弯曲杆菌属的各种培养方法（Skirrow培养基，无血培养基和富含Bolton和Preston肉汤的过滤系统）。从生肉样品中

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We compared six procedures and investigated the optimal method for isolation of Campylobacter spp. from raw meat samples. Ninety-nine meat samples were enriched in Bolton broth and Preston broth, followed by plating on Skirrow, mCCDA, and blood agar (a membrane filter on its surface) media, respectively. Thirty-nine of 99 samples were positive and 71 Campylobacter were isolated by one or more methods. More than one species of Campylobacter were obtained in 8 (20.5?%) of 39 positive samples and two genotypes were yielded on the same medium (11 samples, 28.2?%) by pulsed-field gel electrophoresis (PFGE) genotyping. Enrichment by Preston broth was significantly better than by Bolton broth (P?
机译：我们比较了六个程序，并研究了分离弯曲杆菌属的最佳方法。从生肉样品中提取。在Bolton肉汤和Preston肉汤中富集了99个肉样品，然后分别在Skirrow，mCCDA和血琼脂（其表面的膜过滤器）培养基上铺板。通过一种或多种方法，在99个样品中有39个呈阳性，分离出71个弯曲杆菌。在39个阳性样品中的8个（占20.5％）中获得了一种以上的弯曲杆菌，并且通过脉冲场凝胶电泳（PFGE）基因分型在同一培养基（11个样品，占28.2％）上产生了两种基因型。普雷斯顿肉汤的浓缩作用明显优于博尔顿肉汤（P <0.05）。此外，后者未能检测到空肠弯曲菌菌株。 Skirrow培养基的效率显着低于mCCDA培养基和膜过滤法（P <0.05）。总体而言，PC（在Preston肉汤中进行初次富集，然后在mCCDA琼脂上进行选择性富集），PF（在Preston肉汤中进行初次富集，然后在血琼脂上进行膜过滤培养）和BF（在Bolton肉汤中进行初次富集）相结合通过膜过滤培养到血琼脂上）方法提供了弯曲杆菌属菌的最佳分离率。

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