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首页> 外文期刊>Analytical methods >Direct analysis in real-time high-resolution mass spectrometry as a valuable tool for polyphenols profiling in olive oil
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Direct analysis in real-time high-resolution mass spectrometry as a valuable tool for polyphenols profiling in olive oil

机译：实时高分辨率质谱法中的直接分析是橄榄油中多酚谱分析的重要工具

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A fast and reliable method to characterize the polyphenolic compound profiles in extra virgin olive oil (EVOO) has been developed using direct analysis in real time (DART) and linear ion-trap Orbitrap mass spectrometry (LTQ-Orbitrap-MS). Hydroalcoholic extraction increased speed and reduced matrix effects, and DART-MS/MS ensured accurate analysis. Characterization of polyphenol fingerprinting in EVOO samples takes 2 min. This method exhibited proper linearity (R2 ≥ 0.99) in the range of 5–2500 μg g?1, limit of detection (LOD) of 1.5 μg g?1 (signal-to noise S/N = 3), and limits of quantitation (LOQs) of 5 μg g?1 (S/N = 10) for resveratrol (a polyphenol not detected in olive oil). Six spiked levels of this compound in EVOO were evaluated and the recoveries ranged from 75 to 120%. This approach has demonstrated the capability of generating results comparable to LC-MS results with significantly reduced analysis time. The polyphenols identified by DART-MS in 2 min were detected by LC-MS, but in 45 min. These results demonstrated that DART-Orbitrap was a rapid and simple method which could be applied for rapid analysis of polyphenol fingerprinting in EVOO. Principal component analysis (PCA) filtered out the most pertinent data from the MS spectra and classified the EVOO samples, analyzed according to the olive variety.
机译：使用实时直接分析（DART）和线性离子阱Orbitrap质谱（LTQ-Orbitrap-MS），开发了一种快速可靠的方法来表征特级初榨橄榄油（EVOO）中的多酚类化合物。水醇萃取可提高速度并降低基质效应，而DART-MS / MS可确保准确分析。 EVOO样品中多酚指纹图谱的表征需要2分钟。该方法在5-2500μgg？1的范围内表现出适当的线性度（R2≥0.99），检测限（LOD）为1.5μgg？1（信噪比S / N = 3），以及定量限白藜芦醇（在橄榄油中未检测到的多酚）的（LOQ）为5μgg？1（S / N = 10）。评估了该化合物在EVOO中的六种加标水平，回收率范围为75％至120％。这种方法证明了产生与LC-MS结果相当的结果的能力，同时大大减少了分析时间。通过DART-MS在2分钟内检测到的多酚通过LC-MS检测，但在45分钟内检测到。这些结果表明，DART-Orbitrap是一种快速而简单的方法，可用于快速分析EVOO中的多酚指纹图谱。主成分分析（PCA）从MS质谱图中滤出了最相关的数据，并对EVOO样品进行了分类，并根据橄榄品种进行了分析。

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