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首页> 外文期刊>Analytical methods >Holistic analysis of seven constituents from five medicinal herbs composing Wuhu powders in a single run by ultra performance liquid chromatography: application to quality control study
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Holistic analysis of seven constituents from five medicinal herbs composing Wuhu powders in a single run by ultra performance liquid chromatography: application to quality control study

机译：超高效液相色谱法一次分析芜湖散的五种药材中七种成分的整体分析：在质量控制研究中的应用

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摘要

The newly proposed strategy, namely, holistic analysis of multiple constituents from every constituent herb in a single run, was applied for the quality control of traditional Chinese medicine preparation Wuhu powder. Seven bioactive components, i.e., ferulic acid, hydroxysafflor yellow A, prim-O-glucosylcimifugin, 4a€2-O-?2-D-glucosyl-5-O-methylvisamminol, imperatorin, isoimperatorin, and linoleic acid, from 5 medicinal herbs composing Wuhu powder were selected as evaluation markers, and then their quantitative information was obtained in a single run by an UPLC method coupled with photodiode array detection. Optimum separation was achieved on a C18 column (50 ?— 2.1 mm i.d., 1.7 ??m particle) through a gradient delivery of a mixture of acetonitrile and 0.1% aqueous phosphoric acid at a flow rate of 0.3 mL mina?’1 at 30 ?°C. The corresponding maximum absorption wavelengths were selected as the detection wavelengths for each analyte. For all of the analytes, a good linear relationship (r of 0.999) was obtained between peak area and concentration. The method was validated for repeatability, precision, stability, accuracy, and selectivity. The RSD values of retention time and peak area were 0a€“0.6% and 0.7a€“1.0% for intra-assay, and 0.1a€“1.0% and 1.6a€“1.8% for inter-assay precision. Analysis repeatability and stability (24 h) were all lower than 5.0%. The recovery of the method was 96.1a€“97.7% with a RSD of 2.4a€“4.0%. The validated method was applied to quality control of Wuhu powder from different production batches.
机译：新提出的策略，即一次分析每种成分药草中多种成分的整体方法，被用于中药制剂芜湖散的质量控制。从5种草药中提取出7种生物活性成分，即阿魏酸，羟基红花黄色素A，prim-O-葡萄糖基cimifugin，4a€2-O-？2-D-葡萄糖基-5-O-甲基visamminol，imperatorin，isoimperatorin和亚油酸选择组成芜湖粉的粉尘作为评价指标，然后通过UPLC方法结合光电二极管阵列检测，一次性获得其定量信息。通过在30℃下以0.3 mL min-1的流速梯度输送乙腈和0.1％磷酸水溶液的混合物，在C18柱（50？-2.1 mm内径，1.7？m颗粒）上实现了最佳分离。 ℃。选择相应的最大吸收波长作为每种分析物的检测波长。对于所有分析物，在峰面积和浓度之间都获得了良好的线性关系（r> 0.999）。验证了该方法的可重复性，精密度，稳定性，准确性和选择性。批内分析的保留时间和峰面积的RSD值分别为0a±0.6％和0.7a±1.0％，批间精密度为0.1a±1.0％和1.6a±1.8％。分析的重复性和稳定性（24小时）均低于5.0％。该方法的回收率为96.1a“ 97.7％”，RSD为2.4a“ 4.0％。将验证的方法应用于不同生产批次的芜湖粉质量控制。

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