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首页> 外文期刊>Analytical methods >Holistic analysis of seven constituents from five medicinal herbs composing Wuhu powders in a single run by ultra performance liquid chromatography: application to quality control study
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Holistic analysis of seven constituents from five medicinal herbs composing Wuhu powders in a single run by ultra performance liquid chromatography: application to quality control study

机译：高效液相色谱法一次分析芜湖散的五种药材中七种成分的整体分析：在质量控制研究中的应用

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摘要

The newly proposed strategy, namely, holistic analysis of multiple constituents from every constituent herb in a single run, was applied for the quality control of traditional Chinese medicine preparation Wuhu powder. Seven bioactive components, i.e., ferulic acid, hydroxysafflor yellow A, prim-O-glucosylcimifugin, 4'-O-β-D-glucosyl-5-O-methyivisamminol, imperatorin, isoimperatorin, and linoleic acid, from 5 medicinal herbs composing Wuhu powder were selected as evaluation markers, and then their quantitative information was obtained in a single run by an UPLC method coupled with photodiode array detection. Optimum separation was achieved on a C_(18) column (50 x 2.1 mm i.d., 1.7 μm particle) through a gradient delivery of a mixture of acetonitrile and 0.1% aqueous phosphoric acid at a flow rate of 0.3 mL min~(-1) at 30 °C The corresponding maximum absorption wavelengths were selected as the detection wavelengths for each analyte. For all of the analytes, a good linear relationship (r of >0.999) was obtained between peak area and concentration. The method was validated for repeatability, precision, stability, accuracy, and selectivity. The RSD values of retention time and peak area were 0-0.6% and 0.7-1.0% for intra-assay, and 0.1-1.0% and 1.6-1.8% for inter-assay precision. Analysis repeatability and stability (24 h) were all lower than 5.0%. The recovery of the method was 96.1-97.7% with a RSD of 2.4-4.0%. The validated method was applied to quality control of Wuhu powder from different production batches.
机译：新提出的策略，即一次分析每种成分药草中多种成分的整体方法，被用于中药制剂芜湖散的质量控制。来自芜湖的5种药材中的7种生物活性成分，即阿魏酸，羟基红花黄A，pr​​im-O-葡萄糖基cimifugin，4'-O-β-D-葡萄糖基-5-O-甲基维他命醇，欧前胡素，异欧前胡素和亚油酸选择粉末作为评估标记，然后通过UPLC方法与光电二极管阵列检测相结合，一次获得其定量信息。通过以0.3 mL min〜（-1）的流速梯度输送乙腈和0.1％磷酸水溶液的混合物，在C_（18）色谱柱（50 x 2.1 mm内径，1.7μm颗粒）上实现了最佳分离在30°C下，选择相应的最大吸收波长作为每种分析物的检测波长。对于所有分析物，在峰面积和浓度之间都获得了良好的线性关系（r> 0.999）。验证了该方法的可重复性，精密度，稳定性，准确性和选择性。批内分析的保留时间和峰面积的RSD值分别为0-0.6％和0.7-1.0％，批间分析的精密度为0.1-1.0％和1.6-1.8％。分析的重复性和稳定性（24小时）均低于5.0％。该方法的回收率为96.1-97.7％，RSD为2.4-4.0％。将验证的方法应用于不同生产批次的芜湖粉质量控制。

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