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Assisted reproductive technologies (ART) in water buffaloes

机译：水牛的辅助生殖技术（ART）

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Our expanding knowledge of ovarian function during the buffalo estrous cycle has given new approaches for the precise synchronization of follicular development and ovulation to apply consistently assisted reproductive technologies (ART). Recent synchronization protocols are designed to control both luteal and follicular function and permit fixed-time AI with high pregnancy rates during the breeding (autumn-winter) and nonbreeding (spring-summer) seasons. Additionally, allow the initiation of superstimulatory treatments at a self-appointed time and provide opportunities to do fixed-time AI in donors and fixed-time embryo transfer in recipients. However, due the scarce results of in vivo embryo recovery in superovulated buffaloes, the association of ovum pick-up (OPU) with in vitro embryo production (IVEP) represents an alternative method of exploiting the genetics of high yeld buffaloes. Nevertheless, several factors appear to be critical to OPU/IVEP efficiency, including antral follicle population, follicular diameter, environment, farm and category of donor. This review discusses a number of key points related to the manipulation of ovarian follicular growth to improve assisted reproductive technologies in buffalo.
机译：我们在水牛发情周期中对卵巢功能的不断了解，为卵泡发育和排卵的精确同步提供了新方法，以应用一致的辅助生殖技术（ART）。最近的同步协议旨在控制黄体和卵泡功能，并允许在繁殖季节（秋冬）和非繁殖季节（春夏季）以较高的妊娠率进行固定时间的人工授精。此外，允许在自己指定的时间开始超级刺激治疗，并提供在捐献者中进行固定时间AI和在接受者中进行固定时间胚胎移植的机会。但是，由于在超排卵的水牛中体内胚胎恢复的稀少结果，卵子拾取（OPU）与体外胚胎生产（IVEP）的关联代表了利用高产水牛遗传学的另一种方法。然而，一些因素似乎对OPU / IVEP效率至关重要，包括肛门卵泡数量，卵泡直径，环境，农场和供体类别。这篇评论讨论了与卵巢卵泡生长操纵有关的许多关键点，以改善水牛的辅助生殖技术。

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