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首页> 外文期刊>Animal Reproduction >Leporinus elongatus induced spawning using carp pituitary extract or mammalian GnRH analogue combined with dopamine receptor antagonists
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Leporinus elongatus induced spawning using carp pituitary extract or mammalian GnRH analogue combined with dopamine receptor antagonists

机译：鲤鱼垂体提取物或哺乳动物GnRH类似物与多巴胺受体拮抗剂联合使用，可延长产卵孢子虫的产卵

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Several studies have been developed to support the replacement of the crude carp pituitary extract (CPE) by synthetic products for induced reproduction of South American rheophilic species. However, results have been quite heterogeneous and there is no consensus or a routine use of synthetic products in these species. Thus, the aim of this study was to evaluate the ovulatory process in L. elongatus using different protocols of hormonal induction. Thus, fifteen wild mature females maintained at the Experimental Fish Station, Salto Grande, SP, Brazil were submitted to three different hormonal treatments: CPE (fractioned dose: 0.5 and 5.0 mg kg -1 ); mGnRHa (single dose: 3.5 μg kg -1 ) and mGnRHa (single dose: 5.0 μg kg -1 ). The spawning rate and absolute fecundity were similar among the treatments, but fertility rates were higher for CPE treatment (23.60 ± 9.40) then for mGnRHa treatments (close to or zero zero). Although females ovulated in all treatments, none of them provided viable embryos, showing hatching rates close to zero or zero. Both mGnRHa treatments were more potent for inducing the ovulatory process then CPE treatment, which was evidenced by the fact that the formers showed higher volume density of postovulatory follicles (POF). Accordingly, E 2 and 17α-OHP plasma levels were higher for the mGnRHa treated females compared to the CPE one at the time of ovulation. In this study we confirmed previous scientific evidence that, regardless of whether promoting ovulation, the use of conventional CPE and GnRH doses are not appropriate for some South American migratory species, due to the non-attainment of viable embryos. Moreover, we have brought new information about the relationship between reproductive performance and gonadal steroids concentrations using different hormonal therapies, contributing to understand the reasons for Leporinus elongatus embryo loss in induced spawning.
机译：已经开发了一些研究来支持用合成产品代替粗鲤鱼垂体提取物（CPE），以诱导南美嗜性菌种繁殖。但是，结果却非常不同，在这些物种中尚无共识或常规使用合成产品。因此，本研究的目的是使用不同的荷尔蒙诱导方案来评估长形乳杆菌的排卵过程。因此，在巴西SP Salto Grande的实验鱼站饲养的十五只野生成熟雌性动物接受了三种不同的激素治疗：CPE（分次剂量：0.5和5.0 mg kg -1）; mGnRHa（单剂量：3.5μgkg -1）和mGnRHa（单剂量：5.0μgkg -1）。这些处理之间的产卵率和绝对繁殖力相似，但是CPE处理的受精率（23.60±9.40）高于mGnRHa处理的生育率（接近零或零零）。尽管雌性在所有处理中均排卵，但它们均未提供有生命的胚胎，其孵化率接近零或零。两种mGnRHa处理均比CPE处理更有效地诱导排卵过程，事实是前者显示出较高的排卵后卵泡（POF）密度。因此，与排卵时的CPE相比，经mGnRHa治疗的雌性的E 2和17α-OHP血浆水平更高。在这项研究中，我们证实了先前的科学证据，无论是否促进排卵，常规CPE和GnRH剂量的使用都不适合某些南美移民物种，因为它们无法获得活胚。此外，我们使用不同的激素疗法带来了有关生殖性能和性腺甾体类固醇浓度之间关系的新信息，有助于了解导致产卵的长形孢子菌丢失的原因。

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