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Rapid assay of neopterin and biopterin in urine by wooden-tip electrospray ionization mass spectrometry

机译：木尖电喷雾电离质谱法快速测定尿液中的新蝶呤和生物蝶呤

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摘要

A rapid and sensitive method based on wooden-tip electrospray ionization mass spectrometry (WT-ESI-MS) has been established for the quantitative detection of neopterin and biopterin in urine with only little sample preparation required. The limits of detection (LODs) for the analyses of neopterin and biopterin were determined to be 30 ng mLa?’1 and 50 ng mLa?’1 (S/N a‰￥ 3), respectively. Acceptable relative standard deviation (RSD) values (6.3a€“9.5%) and recovery values (90a€“133.6%) were obtained for direct measurements of neopterin and biopterin in raw urine. Moreover, neopterin and biopterin were directly detected from 9 clinical urine samples by WT-ESI-MS. A single sample analysis was completed within a few minutes, indicating that the present WT-ESI-MS method is a promising strategy for the rapid analysis of clinical samples.
机译：建立了一种基于木尖电喷雾电离质谱（WT-ESI-MS）的快速灵敏的方法，只需少量样品即可定量检测尿液中的新蝶呤和双蝶呤。测定新蝶呤和双蝶呤的分析的检出限（LOD）分别为30 ng mLa？1和50 ng mLa？1（S / N a ￥ 3）。直接测量生尿中新蝶呤和新蝶呤的可接受相对标准偏差（RSD）值（6.3a“ 9.5％）和回收率（90a” 133.6％）。此外，通过WT-ESI-MS直接从9个临床尿液样本中检测到新蝶呤和双蝶呤。几分钟内完成了一次样品分析，表明当前的WT-ESI-MS方法是快速分析临床样品的一种有前途的策略。

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