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首页> 外文期刊>Analytical Sciences >Rapid Detection of the Bursaphelenchus Xylophilus by Denaturation Bubble-mediated Strand Exchange Amplification
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Rapid Detection of the Bursaphelenchus Xylophilus by Denaturation Bubble-mediated Strand Exchange Amplification

机译：变性气泡介导的链交换扩增技术快速检测 Bursaphelenchus xylophilus

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摘要

Bursaphelenchus xylophilus (B. xylophilus ) is one of the most important causal agents of infectious diseases in forest pathology. Obviously, the rapid detection of B. xylophilus is an urgent need for its prevention and cure. We have developed a detection method of B. xylophilus by strand exchange amplification (SEA). This method could detect 10~(5) copies of genomic DNA of B. xylophilus , and it was sufficiently sensitive to detect a single nematode as short as 40 min. Moreover, because the amplification result could be visualized by the naked eyes, the only equipment required throughout the process was a simple isothermal block. Therefore, our method would be a potential for developing on-site detection of B. xylophilus to prevent and control its spread.
机译：嗜酸小球菌（B. xylophilus）是森林病理学中最重要的传染病病因之一。显然，B的快速检测。木糖是其预防和治疗的迫切需要。我们已经开发了 B的检测方法。通过链交换扩增（SEA）的木糖。该方法可检测出10〜（5）个B基因组DNA拷贝。 Xylophilus，它足够灵敏以检测短至40分钟的单个线虫。此外，由于可以通过肉眼看到扩增结果，因此整个过程所需的唯一设备是简单的恒温箱。因此，我们的方法将是开发iB现场检测的潜力。防止和控制木糖的传播。

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