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Transgenic Investigation of Canola (Brassica napus L.)

机译:双低油菜籽(Brassica napus L.)的转基因研究

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Investigating transgenic characteristics of Californium, Jura, Elvis and Orkan varieties of canola has been carried out in this study. These plants are widely cultivated in Turkey. The Canola varieties were grown in an established tissue culture and the total DNA isolated. The 35S and pNOS promoter region of the transgene were scanned using classic and multiplex PCR techniques. The transfer of transgenic characteristic to the NAD gene region confirms the accuracy of the PCR technique. This study, has determined that Californium, Jura, Elvis and Orkan varieties of canola consist of transgenic characteristics and all the four varieties have multiple insertion region in terms of CaMV 35S and Pnos promoters.
机译:在这项研究中,已经调查了卡诺油菜,J菜,猫王和Orkan品种的转基因特性。这些植物在土耳其广泛种植。在已建立的组织培养物中培养油菜品种,并分离总DNA。使用经典和多重PCR技术扫描转基因的35S和pNOS启动子区域。转基因特征向NAD基因区域的转移证实了PCR技术的准确性。这项研究已经确定,双低油菜籽的加利福尼亚,朱拉,猫王和Orkan品种具有转基因特征,并且就CaMV 35S和Pnos启动子而言,所有四个品种均具有多个插入区域。

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