Prestaining Method as a Useful Tool for the Agarose Gel Electrophoretic Detection of Polymerase Chain Reaction Products with a Fluorescent Dye SYBR® Gold Nucleic Acid Gel Stain

Emi SUENAGA; Hiroshi NAKAMURA

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Prestaining Method as a Useful Tool for the Agarose Gel Electrophoretic Detection of Polymerase Chain Reaction Products with a Fluorescent Dye SYBR® Gold Nucleic Acid Gel Stain

机译：预染色方法作为一种有用的工具，可用于荧光染料SYBR®金核酸凝胶染色琼脂糖凝胶电泳检测聚合酶链反应产物

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Three staining methods using SYBR® Gold Nucleic Acid Gel Stain (SYBR Gold) as a fluorescent dye were evaluated for the agarose gel electrophoretic detection of DNA. The methods involve prestain, in-gel stain, and poststain methods. DNA markers and polymerase chain reaction (PCR) products obtained by minisatellite variant repeat-PCR (MVR-PCR) amplification in a D1S8 locus were used as model DNA and practical samples, respectively. Among the three methods tested under the usual electrophoretic conditions, a prestain method using a 10000-fold diluted SYBR Gold solution showed most excellent features regarding cost and rapidity to use with good stainability and resolution over loaded DNA amounts of about 98 ng to 300 ng. The prestain method was found to be applicable to the analysis of DNA in MVR-PCR products from a human hair root.

机译：对于琼脂糖凝胶电泳检测DNA，评估了三种使用SYBR®金核酸凝胶染料（SYBR Gold）作为荧光染料的染色方法。该方法包括染色前，凝胶内染色和染色后方法。通过在D1S8基因座中进行小卫星变异重复PCR（MVR-PCR）扩增获得的DNA标记和聚合酶链反应（PCR）产物分别用作模型DNA和实际样品。在常规电泳条件下测试的三种方法中，使用10000倍稀释SYBR Gold溶液的预染色方法在成本和使用速度方面表现出最出色的功能，在约98 ng至300 ng的负载DNA量上具有良好的染色性和分辨率。发现该染色方法适用于分析人发根的MVR-PCR产物中的DNA。

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《Analytical Sciences》 |2005年第6期|共5页
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Emi SUENAGA; Hiroshi NAKAMURA;
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中图分类分析化学;
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机译：直接DNA染色法和AND啶橙的整合作为替代SAFE荧光染料在琼脂糖凝胶电泳和微核试验中凋亡检测的敏感性增加
2. Fluorescent Detection of Differentially Expressed cDNA Using SYBR Gold Nucleic Acid Gel Stain. [J] . Danielson KG, Kanthala S, Tuli R, Molecular biotechnology . 2004,第1期

机译：使用SYBR金核酸凝胶染料对差异表达的cDNA进行荧光检测。
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4. APPLICATION OF POLYMERASE CHAIN REACTION CHIP FOR NUCLEIC ACID BASED DETECTION OF WATER BORNE PATHOGENS [C] . C.K. YONG, I.K.LAO, R. MURTHY International Conference on Materials for Advanced Technologies . 2007

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6. Thermal denaturation of double-stranded nucleic acids: prediction of temperatures critical for gradient gel electrophoresis and polymerase chain reaction. [O] . G Steger 1994

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7. Prestaining Method as a Useful Tool for the Agarose Gel Electrophoretic Detection of Polymerase Chain Reaction Products with a Fluorescent Dye SYBR Gold Nucleic Acid Gel Stain [O] . Emi SUENAGA, Hiroshi NAKAMURA 2005

机译：诱捕方法作为琼脂糖凝胶电泳检测聚合酶链式反应产物的有用工具，荧光染料SYBR金核酸凝胶染色
8. Rapid Method for Detection and Identification of Flaviviruses by Polymerase ChainReaction and Nucleic Acid Hybridization. (Reannouncement with New Availability Information) [R] . Watts, D. M., Puri, B., Henchal, E. A., 1994

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Prestaining Method as a Useful Tool for the Agarose Gel Electrophoretic Detection of Polymerase Chain Reaction Products with a Fluorescent Dye SYBR® Gold Nucleic Acid Gel Stain

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