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首页> 外文期刊>Analytical Sciences >DNA Analysis Based on the Local Structural Disruption to the Duplexes Carrying a Luminous Lanthanide Complex
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DNA Analysis Based on the Local Structural Disruption to the Duplexes Carrying a Luminous Lanthanide Complex

机译：DNA分析基于局部结构破坏对携带发光镧系元素的双链体的影响。

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摘要

The two DNA conjugates (split probes) carrying a metal chelator form an integrated luminous lanthanide (Ln3+: Tb3+ or Eu3+) complex on the complementary template DNA (target). The luminous property of this Ln3+ complex has been used for DNA assay. The intensity of the luminescence was affected by the local structural disruption caused by one-base mispairing around the complex. Among the mispairings systematically introduced around the Ln3+ center, vicinal mispairings to the center decreased the emission intensity more. This would be a novel nucleobase-discriminating principle, in which the split probes bind the target tightly, yet still retain sequence selectivity.
机译：带有金属螯合剂的两个DNA偶联物（分裂探针）在互补模板DNA（靶标）上形成集成的发光镧系元素（Ln3 +：Tb3 +或Eu3 +）复合物。该Ln3 +复合物的发光特性已用于DNA测定。发光强度受复合物周围一碱基错配引起的局部结构破坏的影响。在Ln3 +中心周围系统引入的错配中，到中心的邻位错配进一步降低了发射强度。这将是一种新颖的区分核碱基的原理，其中分裂探针紧密结合靶标，但仍保留序列选择性。

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