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首页> 外文期刊>Analytical Sciences >High-throughput Total Cupric Ion Reducing Antioxidant Capacity of Biological Samples Determined Using Flow Injection Analysis and Microplate-based Methods
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High-throughput Total Cupric Ion Reducing Antioxidant Capacity of Biological Samples Determined Using Flow Injection Analysis and Microplate-based Methods

机译：流动注射分析和微孔板法测定生物样品的高通量总铜离子还原抗氧化能力

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High-throughput cupric ion reducing antioxidant capacity (CUPRAC) methods were developed for assessment of total antioxidant capacity (TAC) in urine and serum, based on reduction of Cu(II)-neocuproine complex to highly colored Cu(I)-neocuproine complex, measured spectrophotometrically at 450 nm. The reaction time was significantly reduced from 30 to 4 min by application of a calibration compound (uric acid) with kinetic behavior similar to that shown by urine samples. The method was implemented in a microformat (96 well plates) and also in an automatic fashion (flow injection analysis, FIA). A determination throughput value of 288 h~(?1) (microplate method) or of 15 h~(?1) (automatic FIA) was attained. Application of both methods to human serum (SRM 909b, level I) and urines (n = 9) provided TAC values in agreement with those of the end-point batch method.
机译：开发高通量铜离子还原抗氧化能力（CUPRAC）方法，以将Cu（II）-新皮氨酸复合物还原为高度着色的Cu（I）-新皮氨酸复合物，从而评估尿液和血清中的总抗氧化能力（TAC），在450 nm处用分光光度法测量。通过应用校准化合物（尿酸），将反应时间从30分钟显着减少至4分钟，该化合物的动力学行为与尿液样品显示的相似。该方法以微格式（96孔板）和自动方式（流动注射分析，FIA）实施。测定通量值为288 h？（？1）（微孔板法）或15 h？（？1）（自动FIA）。将两种方法应用于人血清（SRM 909b，I级）和尿液（n = 9）可提供与终点分批法一致的TAC值。

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