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首页> 外文期刊>Animal Cells and Systems >Fermented bitter gourd extract differentially regulates lipopolysaccharide-induced cytokine gene expression through nuclear factor-κB and interferon regulatory factor-1
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Fermented bitter gourd extract differentially regulates lipopolysaccharide-induced cytokine gene expression through nuclear factor-κB and interferon regulatory factor-1

机译：苦瓜提取物通过核因子-κB和干扰素调节因子-1差异调节脂多糖诱导的细胞因子基因表达。

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摘要

Bitter gourd is the fruit of a tropical vine in Asia, Africa, and South America where it is commonly used in traditional medicine. Our study tested the effects of a fermented extract of the bitter gourd on the inflammatory activities of the human monocytic leukemia cell line, THP-1. Treatment with the extract resulted in the suppression of phagocytic as well as lipopolysaccharide (LPS)-induced adhesion activity. Interestingly, the LPS-induced expression of matrix metalloproteinase-9 (MMP-9) and tumor necrosis factor-α (TNF-α) was suppressed by the extract while the expression of Interleukin-8 (IL-8) was upregulated. The extract inhibited the LPS-induced activation of p38 mitogen-activated protein kinase (MAPKs) and nuclear factor-κB (NF-κB), both of which are well known to be required for the expression of MMP-9 and TNF-α. In contrast, the expression of interferon regulatory factor (IRF) 1, a transcription factor involved in the expression of IL-8, but not TNF-α, was enhanced by the extract. Suppression of IRF-1 expression resulted in the elimination of the extract's interleukin-8 (IL-8) enhancing effect. These results indicate that the fermented bitter gourd extract has general anti-inflammatory effects, with a differential effect on the expression of cytokines through modulation of NF-κB and IRF-1 activities.
机译：苦瓜是亚洲，非洲和南美热带藤本植物的果实，通常在传统医学中使用。我们的研究测试了苦瓜发酵提取物对人单核细胞白血病细胞系THP-1炎症活动的影响。用提取物处理导致吞噬细胞以及脂多糖（LPS）诱导的粘附活性受到抑制。有趣的是，LPS诱导的基质金属蛋白酶9（MMP-9）和肿瘤坏死因子-α（TNF-α）的表达被提取物抑制，而白介素8（IL-8）的表达却被上调。提取物抑制LPS诱导的p38促丝裂原活化蛋白激酶（MAPKs）和核因子-κB（NF-κB）的活化，众所周知，这两者都是表达MMP-9和TNF-α所必需的。相反，提取物可增强干扰素调节因子（IRF）1的表达，该因子是与IL-8表达有关的转录因子，但与TNF-α无关。 IRF-1表达的抑制导致提取物白介素8（IL-8）增强作用的消除。这些结果表明发酵的苦瓜提取物具有一般的抗炎作用，通过调节NF-κB和IRF-1活性对细胞因子的表达具有不同的作用。

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