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首页> 外文期刊>Annual Research & Review in Biology >Evaluation of Maize Inbred Lines for Iranian maize mosaic virus (IMMV) Resistance
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Evaluation of Maize Inbred Lines for Iranian maize mosaic virus (IMMV) Resistance

机译：玉米自交系对伊朗玉米花叶病毒（IMMV）抗性的评估

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In present study, the putative resistance capacity of thirty five maize inbred lines against Iranian maize mosaic virus (IMMV) was studied. Reaction to IMMV was analyzed under natural field infection and controlled conditions in a greenhouse in 2010 and 2011. In the greenhouse experiments, the maize plants were inoculated at two-leaf stage using the planthopper Laodelphax striatellus . In the field trials, an early sowing cultivation was used to facilitate a higher infection rate. The responses of inbreds to IMMV were assessed by symptom development and ELISA. The rate of natural infection of IMMV in susceptible control (SC704) was about 20%. MO17 showed about 40% infection in the first year. Both field and greenhouse results confirmed that MO17 was more susceptible to IMMV than SC704 and more reliable to be used as susceptible control in future studies of IMMV. Sowing one row of SC704 as vector spreader between every 5 rows of inbred lines caused sufficient vector propagation and virus transmission. Results of both field and greenhouse experiments showed little and no IMMV infection on K1263/1 and K3547/5, respectively. Hence, they were considered as IMMV-resistant. These lines with CIMMYT origin can also be used for production of resistant hybrids. Results showed that resistance to IMMV was not associated with maize maturity because resistance and susceptibility were found in both early and late matured inbred lines. Disease incidence and ELISA values were strongly correlated. Reduced plant height, ear weight and ear diameter and length and delayed silking were observed in plants infected with IMMV.
机译：在本研究中，研究了35个玉米自交系对伊朗玉米花叶病毒（IMMV）的假定抗性。在自然田间感染和受控条件下，于2010年和2011年在温室中分析了对IMMV的反应。在温室试验中，使用飞虱Laodelphax striatellus在两叶阶段接种了玉米植株。在田间试验中，采用早期播种栽培以提高感染率。通过症状发展和ELISA评估近交对IMMV的反应。在易感对照（SC704）中，IMMV的自然感染率约为20％。 MO17在第一年显示出约40％的感染。田间和温室结果均证实，MO17比SC704更易受IMMV侵害，并且在将来的IMMV研究中用作致病性对照更为可靠。在每5行近交系之间播种一行SC704作为传播载体，引起足够的传播和病毒传播。田间试验和温室试验的结果分别显示在K1263 / 1和K3547 / 5上几乎没有IMMV感染。因此，它们被认为是抗IMMV的。这些源自CIMMYT的品系也可用于生产抗性杂种。结果表明，对IMMV的抗性与玉米的成熟度无关，因为在早熟和近熟的自交系中都发现了抗性和敏感性。疾病发生率与ELISA值高度相关。在感染IMMV的植物中，观察到植物高度，穗重，穗直径和长度降低，并且丝化延迟。

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