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首页> 外文期刊>Annual Research & Review in Biology >Study the Effect of Growth Regulators on Micropropagation of Gloriosa superba L. from Seeds and Their Acclimatization
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Study the Effect of Growth Regulators on Micropropagation of Gloriosa superba L. from Seeds and Their Acclimatization

机译：生长调节剂对super藜种子微繁殖及其驯化作用的研究

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Background: G. superb L. is an important medicinal plant of Ayurvedic system of medicine. It is distributed naturally widely throughout the tropics, and worldwide as a pot plant. Due the poor germination and low viability of seeds plant required more time for growing. As seed germination is poor and vegetative propagation is slow, The methods of rapid micropropagation are required. Procedure for seed germination and micropropagation were established. Results: Fresh and healthy seeds were cultured on Murashige & Skoog medium supplemented with different concentration of BAP and GA3. Best seed germination was observed at 1.0 mg/l BAP and 0.5 mg/l GA3 at minimum time of 49 days. MS medium supplemented with 1.5 mg/l BAP+ 0.5 mg/l IAA and 0.5 mg/l kinetin recorded the highest response of shoot multiplication (3.0±0.3) with best shoot length (3.2±0.24 cm). The regenerated shoots were cultured on MS medium. 1.5 IBA and 0.5 NAA mg/l was found to be the best concentration for maximum percent of root initiation. The rooted plantlets were successfully transferred into plastic cups containing sand, peat and soil in the ratio of 1:1:1 and subsequently established in the greenhouse. Thereafter they were reared for three weeks. The survival rate of regenerants was found to be 60%. Conclusion: According to the observations suitable combination of cytokinin and GA3 proved to be effective in the case of seed germination. In shoot cultures of G. superb L. BAP proved to be the effective cytokinin too.
机译：背景：G. superb L.是印度草药医学系统的重要药用植物。它自然地广泛分布于整个热带地区，并作为盆栽植物在全世界范围内传播。由于种子发芽差且种子活力低，植物需要更多的时间才能生长。由于种子发芽差且营养繁殖缓慢，因此需要快速微繁殖的方法。建立了种子发芽和微繁殖的程序。结果：新鲜健康的种子在补充了不同浓度的BAP和GA 3 的Murashige＆Skoog培养基上培养。在49天的最短时间内，BAP 1.0 mg / l和GA 3 0.5 mg / l时种子萌发最佳。补充有1.5 mg / l BAP + 0.5 mg / l IAA和0.5 mg / l激动素的MS培养基记录了最高的枝条繁殖（3.0±0.3）和最佳的枝条长度（3.2±0.24 cm）。再生的芽在MS培养基上培养。发现1.5 IBA和0.5 NAA mg / l是最大浓度的根部引发的最佳浓度。将生根的小植株成功地转移到盛有沙，泥炭和土壤的塑料杯中，该塑料杯的比例为1：1：1，随后在温室中建立。此后，将它们饲养了三个星期。发现再生体的存活率为60％。结论：根据观察，证明细胞分裂素和GA 3 的适当组合对种子萌发是有效的。在出色的G. BAP的芽培养中也被证明是有效的细胞分裂素。

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