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首页> 外文期刊>AoB PLANTS >An effective nutrient medium for asymbiotic seed germination and large-scale in vitro regeneration of Dendrobium hookerianum, a threatened orchid of northeast India
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An effective nutrient medium for asymbiotic seed germination and large-scale in vitro regeneration of Dendrobium hookerianum, a threatened orchid of northeast India

机译：一种有效的营养培养基，用于非共生种子萌发和大规模体外再生铁皮石hook，印度东北濒危的兰花

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Background and aims Dendrobium hookerianum is a rare and threatened epiphytic orchid of northeast India. Prospects for conservation would be strengthened by developing an in vitro method for mass propagation. Seeds are minute and difficult to use directly in the field for this purpose, being non-endospermous with a low nutrient content and dependent on a specific fungus for germination and early seedling development. Although produced in large numbers (2–3 million per capsule), 5 % germinate naturally in the wild. Our objective was to develop a rapid and successful method for in vitro propagation based on an initial in vitro asymbiotic seed germination step that achieved high percentages. Methodology Effects of four different media, i.e. (i) Murashige and Skoog (MS), (ii) Mitra et al., (iii) Knudson (KC) and (iv) Gamborg et al. (B5), were evaluated for large-scale multiplication by asymbiotic seed germination. Seedling leaf number, shoot number, shoot length, root number and root length were scored. After 7–8 months, large numbers of well-rooted plantlets were transferred to a glasshouse in thermocol pots containing compost. Six different composts based on broken brick and charcoal were compared for their ability to support further development over 90 days of hardening. Principal results The fastest and highest percentage seed germination was achieved using MS medium. Seeds on MS medium germinated in 3–4 weeks compared with 7–8 weeks on B5 medium. Seedling development was also superior on MS medium. The inclusion of plant growth regulators was unnecessary. Compost comprising broken brick and charcoal with an upper layer of moss was found to be the most suitable for the survival of transferred plantlets. Ninety per cent survival of plantlets was achieved 90 days after transfer to a glasshouse. Conclusions The use of MS culture medium is well suited for the mass multiplication of D. hookerianum plants intended for re-introducing this threatened orchid into the wild.
机译：背景与目标石D是印度东北部稀有且受威胁的附生兰花。通过开发一种用于体外繁殖的方法，可以加强保护的前景。种子是微小的，难以直接用于田间，因为它是非胚乳，营养成分低，并且依赖于特定的真菌进行发芽和早期幼苗发育。尽管大量生产（每个胶囊2–3百万个），但只有不到5％的种子在野外自然发芽。我们的目标是根据初始的体外非共生种子萌发步骤（达到较高的百分比），开发一种快速成功的体外繁殖方法。方法论四种不同介质的影响，即（i）Murashige和Skoog（MS），（ii）Mitra等人，（iii）Knudson（KC）和（iv）Gamborg等人。 （B 5 ），通过非共生种子萌发评估大规模繁殖。对幼苗叶片数，芽数，芽长，根数和根长进行评分。 7–8个月后，将根深蒂固的小植株转移到装有堆肥的Thermocol盆中的温室中。比较了基于碎砖和木炭的六种不同的堆肥在90天的硬化过程中支持进一步发展的能力。主要结果使用MS培养基获得最快和最高百分比的种子发芽。 MS培养基上的种子在3-4周内发芽，而B 5 培养基上的种子则需要7-8周。在MS培养基上，幼苗的生长也很出色。不需要包括植物生长调节剂。发现包括碎砖和木炭以及上层苔藓的堆肥最适合于转移的幼苗的生存。移入温室后90天，苗的成活率达到90％。结论MS培养基的使用非常适合于D. hookerianum植物的大规模繁殖，该植物旨在将这种濒临灭绝的兰花重新引入野外。

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