...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Asymbiotic seed germination and in vitro seedling development of the threatened orchid Hoffmannseggella cinnabarina
【24h】

Asymbiotic seed germination and in vitro seedling development of the threatened orchid Hoffmannseggella cinnabarina

机译：濒危兰花Hoffmannseggella cinnabarina的非共生种子萌发和离体苗发育

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Hoffmannseggella cinnabarina has not been found in the wild for the last 70 yr in the State of S?o Paulo and, therefore, wild populations of this native orchid are thought to be extinct. This investigation studied seed storage at a low temperature, in vitro germination, and seedling development of H. cinnabarina in order to establish an optimized protocol for propagation, and thus assure species conservation. Seeds of different ages were incubated on Knudson C (KC), Murashige and Skoog, and Vacin and Went media with or without 1 μM of N6-benzyladenine (BA) and exposed to either 12 or 16 h of light (30 μmol?m?2?s?1 at 26?±?2°C). Seed surface sterilization was deleterious to 3-mo-old seeds and severely reduced the viability of the 4-mo-old seeds. More mature seeds were not affected by the sterilization procedure. In general, the germination of 4-, 5-, 6-, and 7-mo-old seeds increased when BA was added to the culture medium especially under 16 h of light. Germination rates were highest with 8- and 9-mo-old seeds, and application of BA failed to enhance germination rates further. Developmental studies revealed that this cytokinin reduced seed and protocorm mortality rates; however, protocorm development was negatively affected in its presence. Seedling development was more pronounced when KC medium with 16 h of light was used. Long-term seed storage at 4°C did not provide promising results. The protocol described in this study proved to be efficient and relevant to in vitro seed germination and initial development of H. cinnabarina, and thus will contribute to conservation of this orchid species.
机译：在圣保罗州，过去70年来没有在野外发现霍夫曼氏菌朱砂，因此，这种原生兰花的野生种群被认为已灭绝。这项研究研究了种子在低温下的贮藏，体外发芽和朱砂树的幼苗发育，以便为繁殖建立优化协议，从而确保物种保护。将不同年龄的种子在Knudson C（KC），Murashige和Skoog以及Vacin和Went培养基上孵育，该培养基可以添加或不添加1μMN6-苄腺嘌呤（BA），并暴露于12或16 h的光照下（30μmol？m？）。在26？±？2°C下为2？s？1）。种子表面灭菌对3个月大的种子有害，并严重降低了4个月大的种子的生存能力。更成熟的种子不受灭菌程序的影响。通常，将BA添加到培养基中时，尤其是在光照16小时的情况下，4、5、6和7个月大种子的发芽增加。 8个月和9个月大的种子发芽率最高，施用BA未能进一步提高发芽率。发育研究表明，这种细胞分裂素降低了种子和原球茎的死亡率。但是，原球茎的发育在其存在下受到负面影响。当使用光照为16 h的KC培养基时，幼苗发育更为明显。种子在4°C下长期保存未获得令人满意的结果。这项研究中描述的协议被证明是有效的，并且与朱砂红花的体外种子萌发和初期发育有关，因此将有助于该兰花物种的保护。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号