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首页> 外文期刊>Antibiotics >Influence of Sterilized Human Fecal Extract on the Sensitivity of Salmonella enterica ATCC 13076 and Listeria monocytogenes ATCC 15313 to Enrofloxacin
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Influence of Sterilized Human Fecal Extract on the Sensitivity of Salmonella enterica ATCC 13076 and Listeria monocytogenes ATCC 15313 to Enrofloxacin

机译：无菌人粪便提取物对肠炎沙门氏菌ATCC 13076和单核细胞增生性李斯特菌ATCC 15313对恩诺沙星敏感性的影响

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There is much debate on whether continuous exposure of commensal bacteria and potential pathogens residing in the human intestinal tract to low levels of antimicrobial agents from treated food animals pose a public health concern. To investigate antimicrobial effects on bacteria under colonic conditions, we studied resistance development in Salmonella enterica and Listeria monocytogenes exposed to enrofloxacin in the presence of fecal extract. The bacteria were incubated at 37 °C in Mueller-Hinton broth, with and without 0.01~0.5 μg/mL enrofloxacin, in the presence and absence of sucrose, and with 1% or 2.5% filter-sterilized fecal extract, for three passages. In the second and third passages, only the bacteria incubated in the media containing sterilized fecal extract grew in 0.5 μg/mL of enrofloxacin. Fecal extract (1% and 2.5%) decreased the sensitivity of S. enterica to enrofloxacin in the medium containing the efflux pump inhibitors reserpine and carbonyl cyanide-m-chlorophenylhydrazone (CCCP) and affected the accumulation of ethidium bromide (EtBr) in this bacterium. Enrofloxacin (0.06 µg/mL) and fecal extract altered the composition of fatty acids in S. enterica and L. monocytogenes. We conclude that fecal extract decreased the susceptibilities of S. enterica and L. monocytogenes to concentrations of enrofloxacin higher than the MIC and resulted in rapid resistance selection.
机译：关于将共生细菌和人类肠道中潜在的病原体持续暴露于来自经过处理的食用动物的低水平抗菌剂中是否引起公众健康的关注，存在许多争议。为了研究结肠条件下对细菌的抗菌作用，我们研究了在粪便提取物存在下暴露于恩诺沙星的肠炎沙门氏菌和单核细胞增生李斯特菌的耐药性发展。在存在和不存在蔗糖的情况下，在有和没有0.01〜0.5μg/ mL恩诺沙星的情况下，将细菌在37°C的Mueller-Hinton肉汤中孵育3遍，分别用1％或2.5％过滤灭菌的粪便提取物进行。在第二和第三次传代中，仅在​​含有无菌粪便提取物的培养基中孵育的细菌在0.5μg/ mL的恩诺沙星中生长。粪便提取物（分别为1％和2.5％）在含有排泄泵抑制剂利血平和羰基氰化物-间氯苯phenyl（CCCP）的培养基中降低了肠炎沙门氏菌对恩诺沙星的敏感性，并影响了该细菌中溴化乙锭（EtBr）的积累。 。恩诺沙星（0.06 µg / mL）和粪便提取物改变了肠炎链球菌和单核细胞增生李斯特氏菌中脂肪酸的组成。我们得出的结论是，粪便提取物降低了肠炎链球菌和单核细胞增生李斯特氏菌对恩洛沙星浓度高于MIC的敏感性，并导致了快速的耐药性选择。

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