• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Antioxidants >The Effect of Sea Buckthorn ( Hippophae rhamnoides L.) Seed Oil on UV-Induced Changes in Lipid Metabolism of Human Skin Cells
【24h】

The Effect of Sea Buckthorn ( Hippophae rhamnoides L.) Seed Oil on UV-Induced Changes in Lipid Metabolism of Human Skin Cells

机译：沙棘籽油对紫外线诱导的人皮肤细胞脂质代谢变化的影响

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Lipids and proteins of skin cells are the most exposed to harmful ultraviolet (UV) radiation contained in sunlight. There is a growing need for natural compounds that will protect these sensitive molecules from damage, without harmful side effects. The aim of this study was to investigate the effect of sea buckthorn seed oil on the redox balance and lipid metabolism in UV irradiated cells formed different skin layers to examine whether it had a protective effect. Human keratinocytes and fibroblasts were subjected to UVA (ultraviolet type A; 30 J/cm 2 and 20 J/cm 2 ) or UVB (ultraviolet type B; 60 mJ/cm 2 and 200 mJ/cm 2 , respectively) radiation and treated with sea buckthorn seed oil (500 ng/mL), and the redox activity was estimated by reactive oxygen species (ROS) generation and enzymaticon-enzymatic antioxidants activity/level (using electron spin resonance (ESR), high-performance liquid chromatography (HPLC), and spectrophotometry). Lipid metabolism was measured by the level of fatty acids, lipid peroxidation products, endocannabinoids and phospholipase A2 activity (GC/MS (gas chromatography/mass spectrometry), LC/MS (liquid chromatography/mass spectrometry), and spectrophotometry). Also, transcription factor Nrf2 (nuclear erythroid 2-related factor) and its activators/inhibitors, peroxisome proliferator-activated receptors (PPAR) and cannabinoid receptor levels were measured (Western blot). Sea buckthorn oil partially prevents UV-induced ROS generation and enhances the level of non-enzymatic antioxidants such as glutathione (GSH), thioredoxin (Trx) and vitamins E and A. Moreover, it stimulates the activity of Nrf2 leading to enhanced antioxidant enzyme activity. As a result, decreases in lipid peroxidation products (4-hydroxynonenal, 8-isoprostaglandin) and increases in the endocannabinoid receptor levels were observed. Moreover, sea buckthorn oil treatment enhanced the level of phospholipid and free fatty acids, while simultaneously decreasing the cannabinoid receptor expression in UV irradiated keratinocytes and fibroblasts. The main differences in sea buckthorn oil on various skin cell types was observed in the case of PPARs—in keratinocytes following UV radiation PPAR expression was decreased by sea buckthorn oil treatment, while in fibroblasts the reverse effect was observed, indicating an anti-inflammatory effect. With these results, sea buckthorn seed oil exhibited prevention of UV-induced disturbances in redox balance as well as lipid metabolism in skin fibroblasts and keratinocytes, which indicates it is a promising natural compound in skin photo-protection.
机译：皮肤细胞的脂质和蛋白质最容易受到阳光中有害的紫外线（UV）的照射。人们越来越需要天然化合物来保护这些敏感分子不受损害，而又没有有害的副作用。这项研究的目的是研究沙棘籽油对形成不同皮肤层的紫外线照射细胞中氧化还原平衡和脂质代谢的影响，以检查其是否具有保护作用。对人角质形成细胞和成纤维细胞进行UVA（A型紫外线; 30 J / cm 2和20 J / cm 2）或UVB（B型紫外线; 60 mJ / cm 2和200 mJ / cm 2）辐射并用沙棘籽油（500 ng / mL），并通过活性氧（ROS）的产生和酶/非酶抗氧化剂的活性/水平（使用电子自旋共振（ESR），高效液相色谱法（ HPLC）和分光光度法）。通过脂肪酸，脂质过氧化产物，内源性大麻素和磷脂酶A2活性（GC / MS（气相色谱/质谱），LC / MS（液相色谱/质谱）和分光光度法）的水平来测量脂质代谢。此外，还测量了转录因子Nrf2（核红系2相关因子）及其激活剂/抑制剂，过氧化物酶体增殖物激活受体（PPAR）和大麻素受体水平（蛋白质印迹）。沙棘油可部分阻止紫外线引起的ROS生成，并提高非酶类抗氧化剂的水平，例如谷胱甘肽（GSH），硫氧还蛋白（Trx）以及维生素E和A。此外，它还能刺激Nrf2的活性，从而增强抗氧化酶的活性。结果，观察到脂质过氧化产物（4-羟基壬烯醛，8-异前列腺素）减少和内源性大麻素受体水平增加。此外，沙棘油处理提高了磷脂和游离脂肪酸的水平，同时降低了紫外线照射的角质形成细胞和成纤维细胞中的大麻素受体表达。在PPAR的情况下，观察到沙棘油在各种皮肤细胞类型上的主要差异-在紫外线照射后的角质形成细胞中，沙棘油处理可降低PPAR的表达，而在成纤维细胞中则观察到相反的作用，表明具有抗炎作用。有了这些结果，沙棘籽油显示出可防止紫外线引起的氧化还原平衡紊乱以及皮肤成纤维细胞和角质形成细胞中的脂质代谢，这表明它是一种有前景的天然皮肤光保护化合物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号