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首页> 外文期刊>Applications in plant sciences. >De Novo Transcriptome Reconstruction of a Thermo-Sensitive Male Sterility Mutant in Rapeseed (Brassica napus; Brassicaceae)
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De Novo Transcriptome Reconstruction of a Thermo-Sensitive Male Sterility Mutant in Rapeseed (Brassica napus; Brassicaceae)

机译：De Novo转录组重建油菜籽（甘蓝型油菜;十字花科）中的热敏雄性不育突变体

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Premise of the study: SP2S is a spontaneous thermo-sensitive genic male sterility (TGMS) mutation that facilitates two-line hybrid breeding in Brassica napus (Brassicaceae). De novo assembly of the floral bud transcriptome of SP2S can provide a foundation for deciphering the transcriptional regulation of SP2S in response to temperature change. Methods: mRNAs of the young floral buds of SP2S and its near-isogenic line SP2F grown under cool (16°C)/warm (22°C) conditions were sequenced on an Illumina Solexa platform, producing 239.7 million short reads with a total length of 19.95 Gbp. Results: The reads were assembled de novo using the Trinity program, resulting in 135,702 transcripts with an average length of 784 bp, an N50 value of 1221 bp, and a total length of 107 Mbp. We identified 24,157 cDNA-derived simple sequence repeats in the assembly. We found 137 and 195 single-nucleotide polymorphisms and 49 and 51 differentially regulated KEGG orthology groups when comparing sample SP2S at 22°C vs. SP2S at 16°C and sample SP2S at 22°C vs. SP2F at 22°C, respectively. Discussion: The numerous differentially expressed genes and the derived single-nucleotide polymorphisms show abnormal transcriptional regulation in the TGMS system. These results outline an intricate transcriptional regulation that occurred in the rapeseed TGMS SP2S when the temperature changed.
机译：研究前提：SP2S是自发的热敏性雄性不育（TGMS）突变，可促进甘蓝型油菜（Brassicaceae）的两系杂交育种。 SP2S花芽转录组的从头组装可以为解密SP2S响应温度变化的转录调控提供基础。方法：在Illumina Solexa平台上对在凉爽（16°C）/温暖（22°C）条件下生长的SP2S及其近等基因系SP2F的年轻花蕾的mRNA进行测序，产生了2.397亿个短读，全长19.95 Gbp。结果：使用Trinity程序从头开始组装读段，得到135,702个转录本，平均长度为784 bp，N50值为1221 bp，总长度为107 Mbp。我们在装配中确定了24,157个cDNA衍生的简单序列重复序列。当分别比较22°C下的SP2S与16°C下的SP2S和22°C下的SP2S与22°C下的SP2F时，我们发现了137个和195个单核苷酸多态性以及49个和51个差异调节的KEGG正交组。讨论：TGMS系统中众多差异表达基因和衍生的单核苷酸多态性显示出异常的转录调控。这些结果概述了温度变化时油菜TGMS SP2S中发生的复杂转录调控。

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