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Transcriptome Sequencing and Marker Development for Four Underutilized Legumes

机译：四种豆类利用不足的转录组测序和标记开发

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Premise of the study: Combating threats to food and nutrition security in the context of climate change and global population increase is one of the highest priorities of major international organizations. Hundreds of species are grown on a small scale in some of the most drought/flood-prone regions of the world and as such may harbor some of the most environmentally tolerant crops (and alleles). Methods and Results: In this study, transcriptomes were sequenced, assembled, and annotated for four underutilized legume crops. Microsatellite markers were identified in each species, as well as a conserved orthologous set of markers for cross-family phylogenetics and comparative mapping, which were ground-truthed on a panel of diverse legume germplasm. Conclusions: An understanding of these underutilized legumes will inform crop selection and breeding by allowing the investigation of genetic variation and the genetic basis of adaptive traits to be established.
机译：研究的前提：在气候变化和全球人口增长的背景下，应对对粮食和营养安全的威胁是主要国际组织的最高优先事项之一。在世界上一些最干旱/最易发生洪灾的地区，数百种物种小规模种植，因此可能藏有一些最耐环境的农作物（和等位基因）。方法和结果：在这项研究中，对四种未充分利用的豆类作物的转录组进行了测序，组装和注释。在每个物种中鉴定出微卫星标记，以及用于跨科系统发育和比较作图的一组保守的直系同源标记，这些标记在各种豆科植物种质上进行了研讨。结论：对这些未充分利用的豆科植物的了解将通过允许对遗传变异和适应性状的遗传基础进行调查，为作物的选择和育种提供依据。

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