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Confirmation of root-knot nematode resistant gene Rmi1 using SSR markers

机译：使用SSR标记确认根结线虫抗性基因Rmi1

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Background: The Root Knot Nematode (RKN) is a serious economic threat to various cultivated crops worldwide. It is a devastating pest of soybean and responsible to cause severe yield loss in Pakistan. The cultivation of resistant soybean varieties against this pest is the sustainable strategy to manage the heavy loss and increase yield. There is an utmost need to identify RKN resistant varieties of soybean against cultivated in Pakistan. The presented study is an attempt to identify and confirm the presence of resistant gene Rmi1 in soybean. Method: Molecular studies have been done using Simple Sequence Repeat (SSR) marker system to identify resistant soybean varieties against Root Knot Nematode (RKN) using fifteen (15) indigenous cultivars and four (4) US cultivars. DNA was isolated, purified, quantified and then used to employ various SSR markers. The amplified product is observed using gel documentation system after electrophoresis. Results: Diagnostic SSR markers Satt-358 and Satt-492 have shown the presence of Rmi1 gene in all resistance carrying genotypes. Satt-358 amplified the fragment of 200 bp and Satt-492 generated 232 bp bands in all resistant genotypes. This study confirmed the Rmi gene locus (G248A-1) in all internationally confirmed resistant including six (6) native varieties. Conclusion: These investigations have identified six (6) resistant cultivars revealing the effective and informative sources that can be utilized in breeding programs for the selection of RKN resistance soybean genotypes in Pakistan.
机译：背景：根结线虫（RKN）对全世界各种栽培作物构成严重的经济威胁。它是大豆的毁灭性害虫，应引起巴基斯坦的严重减产。种植抗这种病虫害的抗性大豆品种是管理沉重损失和增加产量的可持续战略。迫切需要确定针对巴基斯坦种植的抗RKN大豆品种。提出的研究是试图鉴定并确认抗性基因Rmi1在大豆中的存在。方法：已经使用简单序列重复（SSR）标记系统进行了分子研究，以使用十五（15）个本地品种和四（4）个美国品种来鉴定抗根结线虫（RKN）的大豆品种。分离，纯化，定量DNA，然后用于采用各种SSR标记。电泳后，使用凝胶记录系统观察扩增产物。结果：诊断性SSR标记Satt-358和Satt-492已显示在所有带有抗性的基因型中Rmi1基因的存在。 Satt-358扩增了200 bp的片段，Satt-492在所有抗性基因型中产生了232 bp的条带。这项研究在所有国际确认的抗性中证实了Rmi基因位点（G248A-1），包括六（6）个本地变种。结论：这些调查确定了六（6）个抗性品种，揭示了可用于巴基斯坦RKN抗性大豆基因型选择育种计划的有效信息源。

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