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首页> 外文期刊>Advancements in Life Sciences >DNA Barcoding: Amplification and sequence analysis of rbcl and matK genome regions in three divergent plant species
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DNA Barcoding: Amplification and sequence analysis of rbcl and matK genome regions in three divergent plant species

机译：DNA条形码：三种不同植物物种的rbcl和matK基因组区域的扩增和序列分析

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Background: DNA barcoding is a novel method of species identification based on nucleotide diversity of conserved sequences. The establishment and refining of plant DNA barcoding systems is more challenging due to high genetic diversity among different species. Therefore, targeting the conserved nuclear transcribed regions would be more reliable for plant scientists to reveal genetic diversity, species discrimination and phylogeny. Methods: In this study, we amplified and sequenced the chloroplast DNA regions ( matk+rbcl) of Solanum nigrum , Euphorbia helioscopia and Dalbergia sissoo to study the functional annotation, homology modeling and sequence analysis to allow a more efficient utilization of these sequences among different plant species. These three species represent three families; Solanaceae , Euphorbiaceae and Fabaceae respectively. Biological sequence homology and divergence of amplified sequences was studied using Basic Local Alignment Tool (BLAST). Results: Both primers ( matk+rbcl ) showed good amplification in three species. The sequenced regions reveled conserved genome information for future identification of different medicinal plants belonging to these species. The amplified conserved barcodes revealed different levels of biological homology after sequence analysis. The results clearly showed that the use of these conserved DNA sequences as barcode primers would be an accurate way for species identification and discrimination. Conclusion: The amplification and sequencing of conserved genome regions identified a novel sequence of matK in native species of Solanum nigrum. The findings of the study would be applicable in medicinal industry to establish DNA based identification of different medicinal plant species to monitor adulteration.
机译：背景：DNA条形码是一种基于保守序列核苷酸多样性的物种鉴定新方法。由于不同物种之间的高度遗传多样性，植物DNA条形码系统的建立和完善更具挑战性。因此，以保守的核转录区为目标，对于植物科学家来说揭示遗传多样性，物种歧视和系统发育将更为可靠。方法：在本研究中，我们对黑茄，大戟天芥和黄檀的叶绿体DNA区域（matk + rbcl）进行了扩增和测序，以研究功能注释，同源性建模和序列分析，以便更有效地利用这些序列。植物品种。这三个物种代表三个科。茄科，大戟科和豆科。使用基本局部比对工具（BLAST）研究了生物学序列的同源性和扩增序列的差异。结果：两种引物（matk + rbcl）均在三个物种中均表现出良好的扩增效果。测序的区域揭示了保守的基因组信息，以用于将来鉴定属于这些物种的不同药用植物。序列分析后，扩增的保守条形码显示出不同水平的生物学同源性。结果清楚地表明，将这些保守的DNA序列用作条形码引物将是进行物种鉴定和区分的准确方法。结论：保守基因组区域的扩增和测序鉴定出了茄茄天然物种中一个新的matK序列。该研究的结果将可用于医药行业，以建立基于DNA的不同药用植物物种的鉴定，以监测掺假。

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