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Analysis of mRNA Expression for Injury-Age Estimation

机译：损伤年龄估计的mRNA表达分析

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Background: Determination of wound age is pivotal in forensic medical sciences, criminal and civil cases for the construction of crime scene and answering the questions like, time of infliction, manner of wound infliction, how long the person survives after infliction of wound and characterizing antemortem or postmortem wounds. The findings differ considerably among individuals due to the biological variations. Previous developed method in the injury-age determination is clinical, microscopic, enzymatic reaction at the wound margins, histological, and immunohistochemistry with the pitfalls associated with it. Methods: This study is conducted on blunt injuries, particularly lacerated wound (type of wound inflicted by blunt weapon) to analyze the different expression pattern in injury-age up to 72hrs in total 21 individuals randomly grouped in different time intervals. To determine the time of injury, transcript abundance of mRNA of Fibronectin (FBN), IL1β, VEGFA, and GM-CSF was analyzed by real time polymerase chain reaction. 18S-rRNA was used as control marker. Results : Percent knockdown (%KD) was calculated to determine the expression of mRNA for the determination of injury-age. IL1β and GM-CSF showed the predictive behavior for wound age up to 36hrs, Fibronectin (FBN) showed predictive behavior up to 12hrs while VEGFA showed prediction beyond 72hrs. Conclusion: The detection of gradual decrease of mRNA of Fibronectin (FBN), IL1β, VEGFA, and GM-CSF may provide an estimation of wound-age.
机译：背景：确定伤口年龄在法医学，刑事和民事案件中对于犯罪现场的构建至关重要，并回答诸如以下问题：施行时间，施行方式，施行伤亡者的生存时间和定性定性或死后伤口。由于生物学差异，个体之间的发现差异很大。在损伤年龄确定中，先前开发的方法是在伤口边缘进行临床，微观，酶促反应，组织学和免疫组织化学，以及与之相关的缺陷。方法：本研究针对钝伤，特别是划伤的伤口（钝武器造成的伤口类型），分析了在不同时间间隔随机分组的21名个体在长达72小时的损伤年龄中的不同表达方式。为了确定损伤的时间，通过实时聚合酶链反应分析了纤连蛋白（FBN），IL1β，VEGFA和GM-CSF的mRNA的转录本丰度。 18S-rRNA用作对照标记。结果：计算击倒百分比（％KD）以确定mRNA的表达，以确定损伤年龄。 IL1β和GM-CSF在长达36小时的伤口年龄中表现出预测行为，纤连蛋白（FBN）在长达12小时的过程中表现出预测行为，而VEGFA在72小时以上的年龄中表现出预测行为。结论：检测纤连蛋白（FBN），IL1β，VEGFA和GM-CSF的mRNA逐渐降低可能为伤口年龄的估计。

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