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首页> 外文期刊>Advancements in Life Sciences >In vitro anticoccidial activity of Trachyspermum ammi (Ajwain) extract on oocysts of Eimeria species of Chicken
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In vitro anticoccidial activity of Trachyspermum ammi (Ajwain) extract on oocysts of Eimeria species of Chicken

机译：菱草提取物对鸡艾美球虫种卵囊的体外抗球虫活性

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Background: Due to development of synthetic anticoccidial drug resistance there is improvement of anticoccidial medicines due to which exploration of diverse therapeutic agents is attractive now a days. Amongst original agents’ miscellaneous botanicals have shown encouraging properties against coccidiosis. Present study is also a part of probing innovative therapeutic drugs against coccidiosis which can provide replacement solution to treat coccidiosis. Methods: In current experiment in vitro anticoccidial effect of Trachyspermum ammi (seeds) extract was evaluated. For this purpose, an in vitro sporulation inhibition assay was used. Collected oocysts of four Eimeria species were exposed to six different concentrations (w/v) of T. ammi in 10% Dimethyl sulphoxide solution (DMSO), while Dimethyl sulphoxide and Potassium dichromate solution (KSUB2/SUBCrSUB2/SUBOSUB7/SUB) served as control groups. Results: Results of study revealed that T. ammi extract showed in vitro anticoccidial effect by affecting on sporulation (%) and damaging (%) Eimeria oocysts in dose dependent manner. T. ammi extract also damaged the morphology of oocysts in terms of shape, size and number of sporocysts. Conclusion: The results strongly support the botanicals applications of T. ammi extract and also demonstrate its potential for use in Poultry coccidiosis control strategies.
机译：背景：由于合成抗球虫药耐药性的发展，抗球虫药的发展日新月异，因此对多种治疗剂的探索具有吸引力。在原始制剂的其他植物药中，显示出令人鼓舞的抗球虫病特性。目前的研究也是探索针对球虫病的创新治疗药物的一部分，该药物可以为治疗球虫病提供替代解决方案。方法：在目前的实验中，评估了金丝桃（种子）提取物的体外抗球虫作用。为此目的，使用了体外孢子形成抑制测定。在10％的二甲基亚砜溶液（DMSO）中将四种艾美球虫属物种收集的卵囊暴露于六种不同浓度（w / v）的T. ammi，而二甲基亚砜和重铬酸钾溶液（K 2 Cr < SUB> 2 O 7 ）作为对照组。结果：研究结果表明，沙眼衣原体提取物具有剂量依赖性，通过影响孢子虫（％）和破坏（％）艾美球虫卵囊表现出体外抗球虫作用。 T. ammi提取物在孢子囊的形状，大小和数量方面也破坏了卵囊的形态。结论：这些结果有力地支持了棉铃虫提取物的植物学应用，并证明了其在家禽球虫病控制策略中的潜力。

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