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首页> 外文期刊>Archives of Applied Science Research >Effects of Moringa oleifera leaf extract and sodium hypochlorite seed pretreatment on seed germination, seedling growth rate and fungal abundance in two accessions of Abelmoschus esculentus (L) Moench
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Effects of Moringa oleifera leaf extract and sodium hypochlorite seed pretreatment on seed germination, seedling growth rate and fungal abundance in two accessions of Abelmoschus esculentus (L) Moench

机译：辣木叶片提取物和次氯酸钠种子预处理对两种甜菜种子萌发，幼苗生长速率和真菌丰度的影响

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Effects of Moringa oleifera leaf extract and Sodium hypochlorite seed pre-treatment on the seed germination, seedling growth rate and fungal activities in two accessions of Abelmoschus esculentus was examined in the Crop laboratory of Babcock University. Seed viability and seedling vigour were assessed according to ISTA, 1985; while the blotter paper method was used to determine the seed microflora. Mycological analysis was carried out by isolating and identifying fungal flora on potato dextrose agar plates. Okra seeds of CCN2005/2 and Clemson spineless varieties were pre-treated with 2.5%, 5% and 10% of Moringa leaf extract; while Sodium hypochlorite, NaOCl, was administered at 4%, 5% and 6%. A control with no seed pre-treatment was also included. Ten viable seeds, randomly selected from the seed lots of each accession, were dipped into treatment solutions for five minutes, after which it was withdrawn and prepared for further tests. Germination tests were carried out on 85mm diameter petri dishes, lined with moistened Whattman filter paper; with three replications for each treatment in a Completely Randomized Design (CRD). The two okra accessions reacted differently to different concentrations of NaOCl and Moringa leaf extract. The accessions were not significantly different for the seed viability and seedling vigour variables evaluated. Results further reveal that 4% and 6% of NaOCl inhibited the population of fungal growth; while Moringa extract reduced fungal growth and population on both accessions. Fusarium had the highest (67.5%) occurrence on seed fungal population. Penicillium however had the lowest (3.5%). This study explained the fact that pre-treatment of seeds before storage with Moringa reduces the possibility of fungal infection and also maintains the viability and vigour of the seed for a particular time period, depending on the seed type.
机译：在巴比考克大学作物实验室，研究了辣木叶片提取物和次氯酸钠种子预处理对两种种黄花菜种子萌发，幼苗生长速率和真菌活性的影响。种子活力和幼苗活力根据ISTA（1985年）进行评估。吸墨纸法测定种子微生物区系。通过在马铃薯葡萄糖琼脂平板上分离和鉴定真菌菌群来进行真菌学分析。用2.5％，5％和10％的辣木叶提取物预处理CCN2005 / 2和Clemson无脊椎品种的秋葵种子;次氯酸钠（NaOCl）的使用量分别为4％，5％和6％。还包括没有种子预处理的对照。从每个种质的种子批次中随机选择的十个可行种子浸入处理溶液中五分钟，然后将其取出并准备进行进一步测试。在直径为85mm的皮氏培养皿上，用湿的Whattman滤纸作内衬，进行发芽试验。在完全随机设计（CRD）中为每种处理重复三遍。两种秋葵花种对不同浓度的NaOCl和辣木叶提取物的反应不同。所收集的种子活力和幼苗活力变量差异不显着。结果进一步表明，NaOCl的4％和6％抑制了真菌的生长。辣木提取物减少了两种种的真菌生长和种群。镰刀菌在种子真菌种群中的发生率最高（67.5％）。但青霉菌含量最低（3.5％）。这项研究解释了以下事实：根据种子类型，用辣木在储存前进行种子预处理可减少真菌感染的可能性，并在特定时间段内保持种子的生存能力。

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